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| Status |
Public on Aug 23, 2016 |
| Title |
Identification of TWIST1 transcriptoinal targets in the cranial mesoderm [WNT1 vs. MESP1] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
TWIST1, a basic helix-loop-helix transcription factor is essential for the development of cranial mesoderm and cranial neural crest-derived craniofacial structures. Our previous work showed that, in the absence of TWIST1, some cells within the cranial mesoderm adopt an abnormal epithelial configuration. Here, we show by transcriptome analysis that loss of TWIST1 in the cranial mesoderm is accompanied by a reduction in the expression of genes that are associated with cell-extracellular matrix interactions and the acquisition of mesenchymal characteristics. By comparing the transcriptional profiles of cranial mesoderm-specific Twist1 loss-of-function mutant and control mouse embryos, we identified a set of genes that are both TWIST1-dependent and predominantly expressed in the mesoderm. By ChIP-seq in a cell line model of a TWIST1-dependent mesenchymal state, we identified, among the downstream genes, three direct transcriptional targets of TWIST1: Ddr2, Pcolce and Tgfbi. Our findings show that the mesenchymal properties of the cranial mesoderm is likely to be regulated by a network of TWIST1 targets genes that influence the extracellular matrix and cell-matrix interactions, and collectively they are required for the morphogenesis of the craniofacial structures.
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| Overall design |
Cranial neural crest and cranial mesoderm cells were isolated by flow sorting of GFP reporter-labelled cells collected from heads of E9.5 mouse embryos. Three replicates were independently isolated and hybridized to Illumina mouse WG v 2.0 chips
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| Contributor(s) |
Tam P |
| Citation(s) |
27546376 |
| |
| Submission date |
Apr 15, 2016 |
| Last update date |
Jan 16, 2019 |
| Contact name |
PIERRE OSTEIL |
| E-mail(s) |
posteil@cmri.org.au
|
| Phone |
412012956
|
| Organization name |
CMRI
|
| Department |
Embryology
|
| Lab |
Tam's lab
|
| Street address |
214 Hawkesbury Road
|
| City |
WESTMEAD |
| State/province |
NSW |
| ZIP/Postal code |
2145 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (6)
|
| GSM2124732 |
isolated cranial neural crest replicate 1 |
| GSM2124733 |
isolated cranial neural crest replicate 2 |
| GSM2124734 |
isolated cranial neural crest replicate3 |
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| This SubSeries is part of SuperSeries: |
| GSE80663 |
Identification of TWIST1 transcriptional targets in the cranial mesoderm |
|
| Relations |
| BioProject |
PRJNA318628 |
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