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| Status |
Public on May 01, 2016 |
| Title |
Polycomb Ezh2 controls the fate of GABAergic neurons in the embryonic cerebellum |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
Genetic ablation of Ezh2 in Pax7-Cre driven lineages resulted in developmental defects in E13.5 cerebellum. The transcriptional change and H3K27me3 mark change were investigated by RNA-seq and ChIP-seq on H3K27me3.
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| Overall design |
The Ezh2 gene was genetically ablated by crossing Ezh2 flox allele line and Pax7-Cre line mice, which resulted in Ezh2 gene deletion in embryonic cerebellum. To aid the precise dissection of embryonic cerebellum. A Rosa-YFP allele was also breeded along with the Ezh2 flox allele. E13.5 cerebella were dissected, then immediatedly dropped into RNAlater for mRNA abstraction and RNA-seq, or fixed with 1%PFA for H3K27me3 ChIP-seq assay.
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| Contributor(s) |
Feng X, Juan AH, Wang HA, Ko KD, Zare H, Gutierrez-Cruz G, Sartorelli V |
| Citation(s) |
27068104 |
| |
| Submission date |
Apr 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Hossein Zare |
| E-mail(s) |
hzare@mail.nih.gov
|
| Organization name |
NIH
|
| Street address |
5o South Dr #1347
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA318298 |
| SRA |
SRP073224 |
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