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Series GSE79804 Query DataSets for GSE79804
Status Public on Jul 11, 2016
Title A conserved abundant cytoplasmic long noncoding RNA modulates repression of mRNAs by Pumilio proteins in human cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Thousands of long noncoding RNA (lncRNA) genes are encoded in the human genome, and hundreds of them are evolutionary conserved, but their functions and modes of action remain largely obscure. Particularly enigmatic lncRNAs are those that are exported to the cytoplasm, including NORAD - an abundant and highly conserved cytoplasmic lncRNA. Most of the sequence of NORAD is comprised of repetitive units that together contain at least 17 functional binding sites for the two Pumilio homologs in mammals. Through binding to PUM1 and PUM2, NORAD modulates the mRNA levels of their targets, which are enriched for genes involved in chromosome segregation during cell division. Our results suggest that some cytoplasmic lncRNAs function by modulating the activities of RNA binding proteins, an activity which positions them at key junctions of cellular signaling pathways.
Overall design RNA-seq following perturbations of NORAD lncRNA and Pumilio proteins in U2OS cells
Contributor(s) Ulitsky I, Tichon A
Citation(s) 27406171
Submission date Mar 31, 2016
Last update date May 15, 2019
Contact name Igor Ulitsky
Organization name Weizmann Institute of Science
Street address Hertzl St.
City Rehovot
ZIP/Postal code 76100
Country Israel
Platforms (2)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (26)
GSM2103060 Control plasmid transfection for the PUM1/2 OE 1
GSM2103061 Control plasmid transfection for the PUM1/2 OE 2
GSM2103062 siRNA-mediated knockdown of PUM1, replicate 1
BioProject PRJNA317019
SRA SRP072710

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Supplementary file Size Download File type/resource
GSE79804_RAW.tar 2.3 Mb (http)(custom) TAR (of TXT)
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Processed data provided as supplementary file
Raw data are available in SRA

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