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| Status |
Public on Apr 01, 2016 |
| Title |
Differential gene expression in intestinal mouse crypts after loss of KLF5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To determine potential targets of KLF5 we performed RNA-sequencing on jejunal crypts isolated from Klf5-deficient and wildtype mice, using three biological replicates per genotype. We identified 1,480 genes that showed significant differential expression with at least a 2-fold difference between Klf5-deficient and wildtype (q-value ≤ 0.05) (Figure 5A; Supplementary Table 1). Klf5 expression was significantly downregulated 2.7-fold.
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| Overall design |
Comparison of gene expression after loss of KLF5 in the intestine. Isolated jejunal crypts from 3 wildtype and 3 knockout animals were compared.
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| Contributor(s) |
Bell KN, Shroyer NF |
| Citation missing |
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| |
| Submission date |
Mar 31, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Noah F Shroyer |
| E-mail(s) |
noah.shroyer@bcm.edu
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| Organization name |
Baylor College of Medicine
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| Department |
Medicine - Gastroenterology
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| Street address |
1 Baylor Plaza
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| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA316959 |
| SRA |
SRP072673 |
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