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| Status |
Public on Jul 30, 2007 |
| Title |
HDAC-42 modulates acid-induced gene expression in human SEG-1 esophageal adenocarcinoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The role of acid and bile salts in the pathogenesis of esophageal carcinoma arising from Barrett's metaplasia has been well established. Cell proliferation of Barrett's epithelium in response to pulsatile acid exposure has since been confirmed in vivo using endoscopy specimens.
Histone deactylases (HDACs) modulate nucleosomal packaging of DNA, thereby influencing gene transcription and multiple cancer-associated processes. Thus, we conducted microarray analysis to assess the ability of HDAC-42 to modulate key acid-induced changes as well as to impact other genes altered as the normal esophageal epithelium progresses along the metaplasia-dysplasia-esophageal adenocarcinoma continuum.
Keywords: acid-pulsed cells pretreated with HDAC inhibitor or vehicle
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| Overall design |
SEG-1 cells were pretreated for 24 hours with vehicle or HDAC-42, pulsed with media or acidified media (pH 3.5; 20 mins at 37C), replenished with media or HDAC-42, and harvested 6 hours later. Global gene expression analysis was conducted using the human genome chip U133 2.0 Plus.
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| Contributor(s) |
Kresty LA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 31, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Laura A Kresty |
| E-mail(s) |
kresty.1@osu.edu
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| Organization name |
The Ohio State University
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| Department |
Internal Medicine
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| Street address |
CCC Building, Rm 302B
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43201 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (2) |
| GSM197170 |
SEG1_vehicle pretreated_acid pulsed |
| GSM197171 |
SEG1_HDAC42 pretreated_acid pulsed |
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| Relations |
| BioProject |
PRJNA100715 |
