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| Status |
Public on May 27, 2016 |
| Title |
The mutual effect of Staphylococcus epidermidis and Staphylococcus aureus in dual species biofilms as revealed by RNA-Seq |
| Organisms |
Staphylococcus aureus; Staphylococcus epidermidis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We examined the differential gene expression of Staphylococcus epidermidis and Staphylococcus epidermidis in dual species biofilms. Therefore, we performed RNA-Seq on single and dual species biofilms and we compared the gene expression levels in dual species biofilms to those in single species biofilms.
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|
| Overall design |
RNA was extracted from single and dual species biofilms of S. epidermidis ET-024 and S. aureus Mu50, converted to cDNA and RNA-Seq was performed. Three biological replicates were included.
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| |
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| Contributor(s) |
Vandecandelaere I, Van Nieuwerburgh F, Deforce D, Coenye T |
| Citation(s) |
28263995 |
| |
| Submission date |
Mar 24, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ilse Vandecandelaere |
| E-mail(s) |
ilse_vdcandelaere@hotmail.com
|
| Organization name |
Ghent University
|
| Department |
Pharmaceutical Sciences
|
| Lab |
Laboratory of Pharmaceutical Microbiology
|
| Street address |
Ottergemsesteenweg 460
|
| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
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| Platforms (3) |
| GPL17452 |
Illumina HiSeq 2000 (Staphylococcus aureus) |
| GPL21652 |
Illumina HiSeq 2000 (Staphylococcus epidermidis) |
| GPL21653 |
Illumina HiSeq 2000 (Staphylococcus aureus; Staphylococcus epidermidis) |
|
| Samples (9)
|
| GSM2098500 |
S. aureus Mu50 in single species biofilms_replicate M |
| GSM2098501 |
S. aureus Mu50 in single species biofilms_replicate M1 |
| GSM2098502 |
S. aureus Mu50 in single species biofilms_replicate M2 |
| GSM2098503 |
S. epidermidis ET-024 in single species biofilms_replicate E |
| GSM2098504 |
S. epidermidis ET-024 in single species biofilms_replicate E1 |
| GSM2098505 |
S. epidermidis ET-024 in single species biofilms_replicate E2 |
| GSM2098506 |
dual species biofilms of S. aureus Mu50 and S. epidermidis ET-024_replicate EM |
| GSM2098507 |
dual species biofilms of S. aureus Mu50 and S. epidermidis ET-024_replicate EM1 |
| GSM2098508 |
dual species biofilms of S. aureus Mu50 and S. epidermidis ET-024_replicate EM2 |
|
| Relations |
| BioProject |
PRJNA316215 |
| SRA |
SRP072281 |
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