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| Status |
Public on Mar 19, 2016 |
| Title |
IL-31 regulation of transcripts in skin |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Interleukin-31 (IL-31), a T cells derived cytokine which is mainly produced by CD4+ T cells skewed towards Th2 phenotypes. It signals via a heterodimeric receptors composed of IL-31RA and OSMR that is expressed constitutively in epithelial cells and keratinocytes. IL-31 is shown to play a pathogenic role in allergic and inflammatory diseases. Transgenic mice overexpressing IL-31 have a phenotype similar to atopic dermatitis. Here, we studied the role of IL-31 in skin damage by intradermal administration of recombinant IL-31. Notably, IL-31 was sufficient to increase epidermal basal cell proliferation and thickening of the epidermal layer of skin in mice. Analysis of skin transcriptome indicates a significant increase in the transcripts involved in epidermal cell proliferation and pathological skin remodeling. Thus, our study revealed an important role of IL-31 signaling in activating transcriptional programs involved in the pathophysiology of skin diseases.
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|
| Overall design |
mRNA profiles of C57BL/6 mice skin injected with saline and rIL-31 (20µg) via i.d.
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| |
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| Contributor(s) |
Madala SK |
| Citation(s) |
27556734 |
| |
| Submission date |
Mar 18, 2016 |
| Last update date |
Jul 16, 2019 |
| Contact name |
Mario Medvedovic |
| Organization name |
University of Cincinnati
|
| Department |
Department of Environmental Health
|
| Lab |
Laboratory for Statistical Genomics and Systems Biology
|
| Street address |
3223 Eden Av. ML 56
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45267-0056 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
|
| Samples (6)
|
| GSM2094825 |
C57BL/6 mice injected with saline (50ul) for 14 days via i.d._1 |
| GSM2094826 |
C57BL/6 mice injected with saline (50ul) for 14 days via i.d._2 |
| GSM2094827 |
C57BL/6 mice injected with saline (50ul) for 14 days via i.d._3 |
| GSM2094828 |
C57BL/6 mice injected with rIL-31 (20µg/50ul) for 14 days via i.d._1 |
| GSM2094829 |
C57BL/6 mice injected with rIL-31 (20µg/50ul) for 14 days via i.d._2 |
| GSM2094830 |
C57BL/6 mice injected with rIL-31 (20µg/50ul) for 14 days via i.d._3 |
|
| Relations |
| BioProject |
PRJNA315706 |
| SRA |
SRP072041 |
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