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| Status |
Public on Sep 20, 2018 |
| Title |
Effects of diets differing in energy and protein content on the adipose tissue transcriptome of Norwegian Red Heifers |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Effects of heifer feeding regimes on growth characteristics, metabolism, endocrinology, health and subsequent milk yield has been described in several studies. This indicates that nutritional impacts have both short- and long-term effects on the animal’s metabolic regulation. Adipose tissue has an important role in whole-body metabolic regulation, as it is the main site for both lipid synhtesis, storage and mobilization as well as an endocrinologically active organ. However, little is known about bovine adipose metabolism at gene level, and what is done is mostly found in mature cows by means of microarrays. The goal of this study was to apply next-generation RNA sequencing on the adipose tissue of Norwegian Red heifers fed differently, in order to gain knowledge about nutritionally induced regulatory mechanisms in growing dairy cattle. The objectives were to: 1) Describe the differential gene expression in the adipose tissue of heifers fed diets differing in energy and protein content; 2) Describe the long-term changes in adipose gene expression occuring within treatment groups between the experimental feeding period and 6 months after the transition from experimental diets to a similar diet offered to all animals; 3) Identify any post-treatment effects of the experimental feeding as expressed by differential gene expression between treatment groups still present 6 months after the termination of experimental feeding; and 4) To identify candidate genes for further studies on the breeding and feeding for improved feed efficiency and production in the Norwegian Red.
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| Overall design |
The heifers were allocated to 4 experimental treatment groups at 3 months of age; Low-protein/high-energy, high-protein/high-energy, low-protein/low-energy and high-protein/low-energy. The heifers belonged to two different genetic groups, one bred for high milk yield and one bred for low occurence of clinical mastitis. The treatment groups were balanced in this regard. 24 samples were collected at 12 months of age, during the experimental feeding period. There were 6 replicates per treatment group. Transition to a joint pregnancy diet for all animals was performed immediately after confirmed pregnancy (day 28-42 of gestation). New samples were collected from the same animals at month 7 of gestation (20-24 months of age), to give a total of 48 sequenced RNA samples.
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| Contributor(s) |
Lyby Wærp H, Waters SM, McCabe M, Cormican P, Salte R |
| Citation(s) |
30235219, 31269511 |
| |
| Submission date |
Mar 17, 2016 |
| Last update date |
Jul 26, 2019 |
| Contact name |
Hilde Kristine Lyby Wærp |
| E-mail(s) |
hilde.lyby@gmail.com
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| Organization name |
Norwegian University of Life Sciences
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| Department |
Animal and Aquacultural Sciences
|
| Street address |
Arboretveien 6
|
| City |
Aas |
| ZIP/Postal code |
1432 |
| Country |
Norway |
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| Platforms (1) |
| GPL15749 |
Illumina HiSeq 2000 (Bos taurus) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA315560 |
| SRA |
SRP071940 |
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