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Series GSE79248 Query DataSets for GSE79248
Status Public on Mar 16, 2016
Title Type-I interferon inducible genes 26 weeks after mCMV infection in Siglec-H wt and Siglec-H ko mice and their non infected controls
Organism Mus musculus
Experiment type Expression profiling by array
Summary Type I interferons are critical anti-viral cytokines during virus infections and have also been implicated in the pathogenesis of systemic lupus erythematosus (SLE). The secretion of type I interferon of pDCs is modulated by Siglec-H, a DAP12 associated receptor on pDCs. We showed that Siglec-H deficient pDCs produce more of the type I interferon IFN-α in vitro and that Siglec-H ko mice produce more IFN-α after murine cytomegalovirus (mCMV) infection in vivo, leading to efficient clearance of the virus. Furthermore, ageing Siglec-H ko mice showed a mild form of systemic autoimmunity. In contrast, Siglec-H ko mice developed a severe form of systemic lupus-like autoimmune disease with strong kidney nephritis several weeks after a single mCMV infection. This induction of systemic autoimmune disease after virus infection in Siglec-H ko mice was accompanied by a type I interferon signature and fully dependent on type I interferon signaling. These results show that Siglec-H normally serves as modulator of type I interferon responses after infection with a persistent virus and thereby prevents induction of autoimmune disease.
Overall design For microarray experiments gene expression profiles of total splenic cells from two wt and Siglec-H ko mice 26 weeks after infection with luciferase expressing murine Cytomegalovirus (5x105 pfu) or from two uninfected wt and Siglec-H ko control mice were analyzed
Contributor(s) Schmitt H, Sell S, Koch J, Sonnewald S, Daniel C, Winkler TH, Nitschke L
Citation(s) 27377589
Submission date Mar 15, 2016
Last update date Nov 01, 2017
Contact name Heike Schmitt
Phone +4991318528529
Organization name Friedrich Alexander University
Department Biology
Lab Prof. Dr Lars Nitschke
Street address Staudtstraße 5
City Erlangen
ZIP/Postal code 91058
Country Germany
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (8)
GSM2089679 wt 1 non infected
GSM2089680 wt 2 non infected
GSM2089681 ko 1 non infected
BioProject PRJNA315273

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Supplementary file Size Download File type/resource
GSE79248_RAW.tar 17.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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