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Status |
Public on Aug 01, 2017 |
Title |
Determine the function of lncBATE10 in brown and white adipocyte culture |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
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Summary |
Purpose: Determine the function of lncBATE10 in brown and white adipocyte differentiation Methods: primary brown and white apreadipocytes were isolated by cell culture. We infected brown preadipocytes with retroviral shRNA targeting lncBATE10 or with retrovirus overexpressing lncBATE10. Cells were induced to differetinate for 5 days. Total RNA were harvested for RNA-seq Conclusions: Our study shows that lncBATE10 is required for BAT-selective program expression. Overexpression of lncBATE10 is not sufficient to promote BAT marker expression.
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Overall design |
total RNAs from primary brown and white adipocytes cultures (sh-control, shRNA knockdown, overexpression vector, overexpression of lncBATE10) were generated by deep sequencing using Hi-seq 2000
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Contributor(s) |
Sun L, Chai X, Ghosh S |
Citation(s) |
28763438 |
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Submission date |
Mar 14, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Xiaoran Chai |
E-mail(s) |
chaix@gis.a-star.edu.sg
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Organization name |
Genome Institute of Singapore
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Street address |
60 Biopolis Street, Genome, #02-01
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City |
Singapore |
State/province |
Singapore |
ZIP/Postal code |
138672 |
Country |
Singapore |
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Platforms (1) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA317337 |
SRA |
SRP072836 |