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Series GSE79169 Query DataSets for GSE79169
Status Public on Aug 01, 2017
Title Determine the function of lncBATE10 in brown and white adipocyte culture
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Purpose: Determine the function of lncBATE10 in brown and white adipocyte differentiation
Methods: primary brown and white apreadipocytes were isolated by cell culture. We infected brown preadipocytes with retroviral shRNA targeting lncBATE10 or with retrovirus overexpressing lncBATE10. Cells were induced to differetinate for 5 days. Total RNA were harvested for RNA-seq
Conclusions: Our study shows that lncBATE10 is required for BAT-selective program expression. Overexpression of lncBATE10 is not sufficient to promote BAT marker expression.
 
Overall design total RNAs from primary brown and white adipocytes cultures (sh-control, shRNA knockdown, overexpression vector, overexpression of lncBATE10) were generated by deep sequencing using Hi-seq 2000
 
Contributor(s) Sun L, Chai X, Ghosh S
Citation(s) 28763438
Submission date Mar 14, 2016
Last update date May 15, 2019
Contact name Xiaoran Chai
E-mail(s) chaix@gis.a-star.edu.sg
Organization name Genome Institute of Singapore
Street address 60 Biopolis Street, Genome, #02-01
City Singapore
State/province Singapore
ZIP/Postal code 138672
Country Singapore
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM2087175 sh-control
GSM2087176 sh-lncBATE10
GSM2087177 overexpression-control
Relations
BioProject PRJNA317337
SRA SRP072836

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79169_Gene_FPKM.txt.gz 682.7 Kb (ftp)(http) TXT
GSE79169_LncRNA_FPKM.txt.gz 38.5 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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