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| Status |
Public on Sep 01, 2017 |
| Title |
Peptide-signaling and miRNA regulation of transmitter switching affecting social preference |
| Organism |
Xenopus laevis |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Sensory circuit activation can induce neurotransmitter respecification. To understand the consequences and mechanisms of this neuroplasticity we investigated the effects of olfactory system activation on transmitter expression in interneurons of the accessory olfactory bulb (AOB) during development. Frog larvae use olfactory-mediated kin recognition to distinguish siblings from non-siblings. Prolonged exposure to kin (sibling) or non-kin (non-sibling) odorants changed the number of neurons expressing dopamine or GABA compared to odorant deprivation (orphan condition). To identify signaling molecules mediating this behavior we performed mass spectrometry of kin-conditioned water samples. Vitellogenin-derived peptides, uniquely present in kin-conditioned samples of one genotype, were sufficient to elicit aversion behavior in non-kin larvae. RNA profiling identified AOB microRNAs (miRs) differentially regulated across conditions. Inhibition of miR-375 and miR-200b revealed that they regulate the dopaminergic and GABAergic phenotypes by targeting Pax6 and Bcl11b. Altering the ratio of dopamine/GABA AOB interneurons or locally introducing receptor blockers reversed kinship preference.
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| Overall design |
The AOBs of 15 larvae (stage 45) raised in sibling, orphan, or non-sibling conditions were frozen, cryosectioned at 50 μm and neuronal tissue was harvested with scalpels for clean dissection. Total RNA was extracted from tissue with the “Recover All Total Nucleic Acid” isolation kit. RNA quality was assessed on a Bioanalyzer and degraded samples excluded from RNA sequencing. Library preparation and RNA sequencing were performed at the UCSD Biogem core.
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| Contributor(s) |
Dulcis D, Lippi G, Stark CJ, Do LH, Berg DK, Spitzer NC |
| Citation(s) |
28867550 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 NS015918 |
Development of Neurons |
THE REGENTS OF THE UNIV. OF CALIF., UNIV. OF CALIF., SAN DIEGO |
NICHOLAS CANADAY SPITZER |
| R01 NS057690 |
Analysis of Activity Dependent Mechanisms of Neuronal Differentiation |
THE REGENTS OF THE UNIV. OF CALIF., UNIV. OF CALIF., SAN DIEGO |
Davide Dulcis |
| R01 NS057690 |
Analysis of Activity Dependent Mechanisms of Neuronal Differentiation |
THE REGENTS OF THE UNIV. OF CALIF., UNIV. OF CALIF., SAN DIEGO |
NICHOLAS CANADAY SPITZER |
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| Submission date |
Mar 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Giordano Lippi |
| E-mail(s) |
glippi@ucsd.edu
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| Organization name |
University of California, San Diego
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| Department |
Neurobiology
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| Lab |
Darwin Berg
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0649 |
| Country |
USA |
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| Platforms (1) |
| GPL17682 |
Illumina HiSeq 2000 (Xenopus laevis) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA314143 |
| SRA |
SRP071089 |
