GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE78832 Query DataSets for GSE78832
Status Public on Apr 25, 2016
Title Ultraefficient irCLIP platform for characterization of protein-RNA interactions
Organism Homo sapiens
Experiment type Other
Summary The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce an advanced UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, nonisotopic method for the detection of protein-RNA interactions using 100-fold less material than standard protocols.
Overall design Nonisotopic interrogation of in vivo RNA-protein interactions
Contributor(s) Zarnegar BJ, Flynn RA, Shen Y, Do BT, Chang HY, Khavari PA
Citation(s) 27111506
Submission date Mar 02, 2016
Last update date May 15, 2019
Contact name Ryan Alexander Flynn
Organization name Stanford University
Street address 380 Roth Way, Room 265
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM2078373 hnRNP-C irCLIP - 20K cells - Rep1 and 2
GSM2078374 hnRNP-C irCLIP - 100K cells - Rep1 and 2
GSM2078375 HuR irCLIP - 100K cells - Rep1 and 2
BioProject PRJNA314085
SRA SRP071075

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE78832_RAW.tar 9.1 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap