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Series GSE7819 Query DataSets for GSE7819
Status Public on May 25, 2007
Title Comparison of gene expression in SVG-A glial cells and SVGR2 glial cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary SVGR2 cells are glial cells which are derived from SVG-A cells. They were created by subjecting SVG-A cells to multiple rounds of lytic infection by the human polyomavirus JCV. SVGR2 cells are the cells that survived this process and are resistant to JCV infection. This experiment was designed to identify gene expression differences that may be responsible for SVGR2 resistance to JCV.
Keywords: Cell type
Overall design SVG-A and SVGR2 cells are transformed cell lines. To eliminate gene expression differences that may have occurred due to genetic instability of these cell lines three subclones of each of the cell lines were created and used as a triplicate in the comparison. Total RNA was extracted from cells that had been left at 100% confluency for one day using the Qiagen RNeasy kit. cDNA was hybridized to the Affymetrix Human GeneChips U133A or U133B according to Affymetrix gudielines.
Contributor(s) Atwood WJ, Manley K
Citation(s) 17555786
Submission date May 16, 2007
Last update date Aug 10, 2018
Contact name Kate Manley
Organization name Brown University
Street address 70 Ship St.
City Providence
State/province RI
ZIP/Postal code 02903
Country USA
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL97 [HG-U133B] Affymetrix Human Genome U133B Array
Samples (12)
GSM189708 SVG-A subclone 1-D4 GeneChip U133A
GSM189709 SVG-A subclone 3-D6 GeneChip U133A
GSM189710 SVG-A subclone 4-G5 GeneChip U133A
BioProject PRJNA100049

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Supplementary file Size Download File type/resource
GSE7819_RAW.tar 101.5 Mb (http)(custom) TAR (of CEL, CHP, EXP)
Processed data included within Sample table
Processed data provided as supplementary file

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