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| Status |
Public on May 25, 2007 |
| Title |
Comparison of gene expression in SVG-A glial cells and SVGR2 glial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
SVGR2 cells are glial cells which are derived from SVG-A cells. They were created by subjecting SVG-A cells to multiple rounds of lytic infection by the human polyomavirus JCV. SVGR2 cells are the cells that survived this process and are resistant to JCV infection. This experiment was designed to identify gene expression differences that may be responsible for SVGR2 resistance to JCV.
Keywords: Cell type
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| Overall design |
SVG-A and SVGR2 cells are transformed cell lines. To eliminate gene expression differences that may have occurred due to genetic instability of these cell lines three subclones of each of the cell lines were created and used as a triplicate in the comparison. Total RNA was extracted from cells that had been left at 100% confluency for one day using the Qiagen RNeasy kit. cDNA was hybridized to the Affymetrix Human GeneChips U133A or U133B according to Affymetrix gudielines.
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| Contributor(s) |
Atwood WJ, Manley K |
| Citation(s) |
17555786 |
| |
| Submission date |
May 16, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Kate Manley |
| Organization name |
Brown University
|
| Street address |
70 Ship St.
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02903 |
| Country |
USA |
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| Platforms (2) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
| GPL97 |
[HG-U133B] Affymetrix Human Genome U133B Array |
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| Samples (12)
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| GSM189711 |
SVGR2 subclone 1-C4 GeneChip U133A |
| GSM189712 |
SVGR2 subclone 4-E12 GeneChip U133A |
| GSM189713 |
SVGR2 subclone 4-F6 GeneChip U133A |
| GSM189714 |
SVG-A subclone 1-D4 GeneChip U133B |
| GSM189715 |
SVG-A subclone 3-D6 GeneChip U133B |
| GSM189716 |
SVG-A subclone 4-G5 GeneChip U133B |
| GSM189717 |
SVGR2 subclone 1-C4 GeneChip U133B |
| GSM189718 |
SVGR2 subclone 4-E12 GeneChip U133B |
| GSM189719 |
SVGR2 subclone 4-F6 GeneChip U133B |
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| Relations |
| BioProject |
PRJNA100049 |
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