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Status |
Public on Sep 24, 2018 |
Title |
Stella prevents excessive de novo DNA methylation during mouse oogenesis |
Organisms |
Homo sapiens; Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Methylation profiling by high throughput sequencing
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Summary |
Oocyte acquires developmental competence during its maturation. This stage is accompanied with large-scale alteration in transcription, and series of genome-wide epigenetic reprogramming, including de novo establishment of DNA methylation. However, our understanding of mechanisms regulating this process is limited. To investigate the role of Stella (Dppa3) in de novo methylation during mouse oogenesis, here we measured DNA methylation by RRBS and expression profiles by RNA-seq in PGCs and oocytes at serveral development stages, including genotypes of both Stella (Dppa3) +/- and Stella -/-.
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Overall design |
We measured DNA methylation by RRBS and expression profiles by RNA-seq in PGCs and oocytes at serveral development stages (E13.5, D5, D10, D20, MII), including genotypes of both Stella (Dppa3) +/- and Stella -/-. DNA methylation of sperm with both genotypes were also measured by RRBS for comparision.
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Contributor(s) |
Li Y, Zhang Z, Chen J, Huang H, Li X, Xu S, Dong Q, Tan J, Zheng Y, Xu G, Wang Z, Wang H, Zhu B |
Citation(s) |
30487604 |
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Submission date |
Feb 22, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Zhuqiang Zhang |
E-mail(s) |
zhangzhuqiang@ibp.ac.cn
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Organization name |
Institute of Biophysics, CAS
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Street address |
15 Datun Road, Chaoyang District.
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City |
Beijing |
State/province |
--- |
ZIP/Postal code |
100101 |
Country |
China |
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Platforms (4)
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GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (83)
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Relations |
BioProject |
PRJNA312776 |
SRA |
SRP070645 |