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| Status |
Public on Feb 20, 2016 |
| Title |
Silcening YWHAE gene expression suppresses the proliferation of colon cancer cells by inducing gene differentially expressed. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study,YWHAE expression was silenced by RNA interference in colon cancer cell lines, and investigate the correlation between YWHAE expression and colon cancer proliferation,and differential expressed genes were analized by gene assay and qPCR technologe.The proliferation and colony formation ability of colon cancer cells were significantly reduced after YWHAE knockdown. More 1200 known genes were found to be involved in the YWHAE functions related tumorigenesis by genechip analysis, these genes related to cell proliferation/apoptosis and cell cycle process. qRT-PCR results showed an expression pattern consistent with that of the genechip analysis.
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| Overall design |
Lentivirus-mediated RNA interference was used to knockdown YWHAE expression in colon cancer cell lines. And cell proliferation assays . GeneChip analysis were carried out for mRNA expression profiling, followed, the expressed levels of mRNA and protein were measured by Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot , respectively.
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| Contributor(s) |
Huang YS, Xie N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 19, 2016 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Yousheng Huang |
| E-mail(s) |
hys768811@163.com
|
| Organization name |
Hainan Medical university
|
| Department |
Department of pathology
|
| Street address |
The 3rd Xueyuan Road
|
| City |
Haikou |
| State/province |
Hainan |
| ZIP/Postal code |
570102 |
| Country |
China |
| |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
|
| Samples (6)
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| GSM2066595 |
human colon cancer sw480 cells, control shRNA rep1 [re-analysis] |
| GSM2066596 |
human colon cancer sw480 cells, control shRNA rep2 [re-analysis] |
| GSM2066597 |
human colon cancer sw480 cells, control shRNA rep3 [re-analysis] |
| GSM2066598 |
human colon cancer sw480 cells, YWHAE shRNA rep1 |
| GSM2066599 |
human colon cancer sw480 cells, YWHAE shRNA rep2 |
| GSM2066600 |
human colon cancer sw480 cells, YWHAE shRNA rep3 |
|
| Relations |
| BioProject |
PRJNA312593 |
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