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Series GSE77962 Query DataSets for GSE77962
Status Public on Feb 07, 2017
Title Adipose tissue gene expression is differentially regulated with different rates of weight loss in overweight and obese humans
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background: Moderate weight loss can ameliorate adverse health effects associated with obesity, reflected by an improved adipose tissue (AT) gene expression profile. However, the effect of rate of weight loss on the AT transcriptome is unknown.

Objective: We investigated the global AT gene expression profile before and after two different rates of weight loss that resulted in similar total weight loss, and after a subsequent weight stabilization period.

Design: In this randomized controlled trial, 25 male and 28 female individuals (BMI: 28-35 kg/m2) followed either a low-calorie diet (LCD; 1250 kcal/d) for 12 weeks or a very-low-calorie diet (VLCD; 500 kcal/d) for 5 weeks (weight loss (WL) period) and a subsequent weight stable (WS) period of four weeks. The WL period and WS period together is termed dietary intervention (DI) period. Abdominal subcutaneous AT biopsies were collected for microarray analysis, and gene expression changes were calculated for all three periods in the LCD group, VLCD group and between diets (ΔVLCD-ΔLCD).

Results: Weight loss was similar between groups during the WL period (LCD: -8.1±0.5 kg, VLCD: -8.9±0.4 kg, difference p=0.25). Overall, more genes were significantly regulated and changes in gene expression were more extreme in the VLCD group compared to the LCD group. Gene sets related to mitochondrial function, adipogenesis and immunity/inflammation were more strongly upregulated on a VLCD compared to a LCD during the DI period (positive ΔVLCD-ΔLCD). Neuronal- and olfactory-related gene sets were decreased during the WL period and DI period in the VLCD group.

Conclusions: The rate of weight loss (LCD vs. VLCD), with similar total weight loss, strongly regulates AT gene expression. Increased mitochondrial function and adipogenesis on a VLCD compared to a LCD reflect potential beneficial diet-induced changes in AT, while differential neuronal and olfactory regulation suggest functions of these genes beyond the current paradigm.
 
Overall design In this randomized controlled trial, 53 male and female individuals (BMI: 28-35 kg/m2) underwent a dietary intervention (DI) with two different rates of weight loss consisting of either a low-calorie diet (1250 kcal/d) for 12 weeks or a very-low-calorie diet (500 kcal/d) for 5 weeks (weight loss (WL) period) and a subsequent weight stable (WS) period of four weeks. Abdominal subcutaneous AT biopsies were collected for whole-genome gene expression analysis at study start, after the WL period and after the WS period.

This trial is registered with www.clinicaltrials.gov under the accession number NCT01559415.
 
Contributor(s) Vink RG, Roumans NJ, Fazelzadeh P, Tareen SH, Boekschoten MV, Mariman EC, van Baak MA
Citation(s) 30380678, 27840413, 32015415
Submission date Feb 16, 2016
Last update date Feb 18, 2020
Contact name Guido Hooiveld
E-mail(s) guido.hooiveld@wur.nl
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
 
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (152)
GSM2062466 Abdominal subcutaneous white adipose tissue, at study start, subject 1
GSM2062467 Abdominal subcutaneous white adipose tissue, after weight loss period, subject 1
GSM2062468 Abdominal subcutaneous white adipose tissue, after weight stable period, subject 1
Relations
BioProject PRJNA312210

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77962_RAW.tar 650.7 Mb (http)(custom) TAR (of CEL)
GSE77962_study_design.pdf 170.7 Kb (ftp)(http) PDF
Processed data included within Sample table

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