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| Status |
Public on Sep 30, 2017 |
| Title |
RNA-SEQ analysis of postnatal Ret-positive (EGFP-labeled) nociceptive (NaV1.8 population) dorsal root ganglia L345 or L6S1 neurons. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this set is to build expression data using FACS isolated Ret-positive cells (postnatal) harvested from a RetFloxedEGFP reporter mouse crossed with NaV1.8Cre mice to enrich for Ret nociceptor population. To enrich for bladder projecting neurons L6S1 DRGs were FACS isolated. L3,4, 5 lumbar levels were also FACS isolated to aid in differential expression of Ret-nociceptor population from L6S1 and L345 spinal DRG levels. The series further includes these populations from males and females to uncover sex based differences.
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| Overall design |
RNA was extracted from 3 month FACS isolated dorsal root ganglia dissected from RetFloxedEGFP (RET9 allele is floxed followed by conditional expression of EGFP: abbreviated 9CG) C57BL/6J mice (knocked-in Ret locus-heterozyotes, EGFP is expressed only after Cre-mediated excision from NaV1.8 cells: in DRGS, only small unmyelinated Ret expressing cells) and sequenced using Illumina Hiseq 2500 platform.
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| Contributor(s) |
Tycksen E, Hoshi M, Golden J, Knoten A, Jain S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 12, 2016 |
| Last update date |
Jul 25, 2021 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
gudmap-db@gudmap.org
|
| Phone |
+44 131 651 8500
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| Organization name |
IGMM MRC Human Genetics Unit
|
| Lab |
GUDMAP Database Group
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA311961 |
| SRA |
SRP070074 |
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