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| Status |
Public on Jul 01, 2016 |
| Title |
Impact of mutations in rv2887, putative transcriptional repressor, on gene expression in Mycobacterium tuberculosis. |
| Organism |
Mycobacterium tuberculosis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study was aimed towards understanding the consequences of mutating rv2887, a putative transcriptional repressor, on gene expression in Mycobacterium tuberculosis. Since clones carrying mutations in this gene were found to be highly resistant to a potent mycobactericidal compound, 14, we also tested the impact of short-term exposure to this compound on gene expression in Mycobacterium tuberculosis.
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| Overall design |
Three different strains, wild type H37Rv and the two clones resistant to compound 14, named 1A and 8A, were grown to log phase in 7H9 complete medium. The cells were diluted to an OD of 0.5 and split in to two equal parts, 1 arm received the vehicle (DMSO) and the other arm received compound 14 at a final concentration of 3.9 μM. The exposure lasted 4 hours, at which point the bacterial pellet was collected and processed for RNA extraction using protocols described previously.
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| Contributor(s) |
Warrier T, Zhang T, Nathan C |
| Citation(s) |
27432954 |
| |
| Submission date |
Feb 03, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Carl Nathan |
| E-mail(s) |
cnathan@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Street address |
1300 York Ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL21405 |
Illumina HiSeq 1000 (Mycobacterium tuberculosis) |
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| Samples (6)
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| GSM2054451 |
wild type strain, H37Rv, treated with vehicle control (DMSO) |
| GSM2054452 |
wild type strain, H37Rv, treated with 3.9 μM of compound 14 |
| GSM2054453 |
resistant clone 1A treated with vehicle control (DMSO) |
| GSM2054454 |
resistant clone 1A treated with 3.9 μM of compound 14 |
| GSM2054455 |
resistant clone 8A treated with vehicle control (DMSO) |
| GSM2054456 |
resistant clone 8A treated with 3.9 μM of compound 14 |
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| Relations |
| BioProject |
PRJNA310781 |
| SRA |
SRP069259 |
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