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| Status |
Public on Oct 07, 2016 |
| Title |
AML1/ETO accelerates cell migration and impairs cell-to-cell adhesion and homing of hematopoietic stem/progenitor cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The AML1/ETO fusion protein found in acute myeloid leukemias functions as a transcriptional regulator by recruiting co-repressor complexes to its DNA binding site. In order to extend the understanding of its role in preleukemia, we expressed AML1/ETO in a murine immortalized pluripotent hematopoietic stem/progenitor cell line, EML C1, and found that genes involved in functions such as cell-to-cell adhesion and cell motility were among the most significantly regulated as determined by RNA sequencing. In functional assays, AML1/ETO-expressing cells showed a decrease in adhesion to stromal cells, an increase of cell migration rate in vitro, and displayed an impairment in homing and engraftment in vivo upon transplantation into recipient mice. Our results suggest that AML1/ETO expression determines a more mobile and less adherent phenotype in preleukemic cells, therefore altering the interaction with the hematopoietic niche, potentially leading to the migration across the bone marrow barrier and to disease progression.
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| Overall design |
RNA-sequencing of EML1 cell line after overexpression of AML1/ETO by retroviral cDNA transduction. Empty (No cDNA) vector was used as control.
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| Contributor(s) |
Saia M, Termanini A, Rizzi N, Mazza M, Barbieri E, Valli D, Ciana P, Alcalay M |
| Citation(s) |
27713544 |
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| Submission date |
Jan 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Alberto Termanini |
| E-mail(s) |
termanini@me.com
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| Organization name |
Istituto Clinico Humanitas
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| Lab |
Bioinformatic Unit
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| Street address |
Via Manzoni, 113
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| City |
Rozzano |
| State/province |
MI |
| ZIP/Postal code |
20089 |
| Country |
Italy |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA309670 |
| SRA |
SRP068841 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE77185_gene_exp.diff.gz |
7.0 Mb |
(ftp)(http) |
DIFF |
| GSE77185_genes.fpkm_tracking.gz |
1.6 Mb |
(ftp)(http) |
FPKM_TRACKING |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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