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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 03, 2016 |
| Title |
Expression data from thymic and lymph node mesenchymal stromal subsets. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Despite their key role in immunity our understanding of primary and secondary lymphoid stromal cell heterogeneity and ontogeny remains limited. Here, using genome-wide expression profiling and phenotypic and localization studies, we identify a functionally distinct subset of BP3-PDPN+PDGFRβ+/α+CD34+ stromal adventitial cells in both lymph nodes and thymus that is located within the perivascular niche surrounding PDPN-PDGFRβ+/α-Esam-1+ITGA7+ pericytes. In re-aggregate organ grafts adult CD34+ adventitial cells gave rise to multiple thymic and lymph node mesenchymal subsets including pericytes, FRC-, MRC- and FDC-like cells, the development of which was lymphoid environment dependent. During thymic ontogeny pericytes developed from a transient population of BP3-PDPN+PDGFRβ+/α+CD34-/lo anlage-seeding progenitors that subsequently up-regulated CD34 and we provide evidence suggesting that similar embryonic progenitors give rise to lymph node mesenchymal subsets. These findings extend the current understanding of lymphoid mesenchymal cell heterogeneity and highlight a role of the CD34+ vascular adventitia as a potential ubiquitous source of lymphoid stromal precursors in postnatal tissues.
To comprehensively study the differences and similarities between mesenchymal stromal subsets in the thymus and lymph nodes, global gene expression analysis was performed on sorted PDPN-, BP-3-PDPN+ and BP-3+PDPN+ PDGFRb+ lymph node mesenchymal cells (LNMC) as well as PDPN- and BP-3-PDPN+ PDGFRb+ thymic mesenchymal cells (TMC) from 2 w old mice by microarray.
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| Overall design |
Total RNA was prepared from TMC and LNMC (pooled inguinal, brachial and axillary LN) subsets sorted from 3 (TMC) and 10-11 (LNMC) 2 weeks old mice per experiment. Isolated RNA from 3 individual experiments was amplified and prepared for hybridization to the Affymetrix Mouse Gene 1.1 ST Array at a genomics core facility: Center of Excellence for Fluorescent Bioanalytics (KFB, University of Regensburg, Germany)
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| Contributor(s) |
Sitnik KM, Weishaupt H, Agace WW |
| Citation(s) |
26947077 |
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| Submission date |
Jan 19, 2016 |
| Last update date |
Apr 18, 2017 |
| Contact name |
William Winston Agace |
| Organization name |
Technical University of Denmark
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| Department |
National Veterinary Institute, Section for Immunology and Vaccinology
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| Street address |
Bülowsvej 27
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| City |
Fredriksberg C |
| ZIP/Postal code |
1870 |
| Country |
Denmark |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (15)
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| GSM2041749 |
PDPN- TMC, biological rep1 |
| GSM2041750 |
PDPN- TMC, biological rep2 |
| GSM2041751 |
PDPN- TMC, biological rep3 |
| GSM2041752 |
PDPN- LNMC, biological rep1 |
| GSM2041753 |
PDPN- LNMC, biological rep2 |
| GSM2041754 |
PDPN- LNMC, biological rep3 |
| GSM2041755 |
BP3+PDPN+ LNMC, biological rep1 |
| GSM2041756 |
BP3+PDPN+ LNMC, biological rep2 |
| GSM2041757 |
BP3+PDPN+ LNMC, biological rep3 |
| GSM2041758 |
BP3-PDPN+ LNMC, biological rep1 |
| GSM2041759 |
BP3-PDPN+ LNMC, biological rep2 |
| GSM2041760 |
BP3-PDPN+ LNMC, biological rep3 |
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| Relations |
| BioProject |
PRJNA309163 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE76974_RAW.tar |
61.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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