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| Status |
Public on Jun 11, 2007 |
| Title |
Cannabinoid receptor double knockout mice (Cnr1 -/- /Cnr2 -/-) in CHS model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We evaluated cutaneous contact hypersensitivity (CHS) in Cnr1-/-/Cnr2-/- animals using the obligate contact allergen 2,4-dinitrofluorobenzene (DNFB), which generates a specific cutaneous T-cell mediated allergic response upon repeated allergen contact. Allergic contact dermatitis affects about 5% of men and 11% of women in industrialized countries and is one of the leading causes for occupational diseases. In an animal model for cutaneous contact hypersensitivity we show that mice lacking both known cannabinoid receptors display exacerbated allergic inflammation. In contrast, fatty acid amide hydrolase deficient mice, which have increased levels of the endocannabinoid anandamide, displayed reduced allergic responses in the skin. Cannabinoid receptor antagonists exacerbated whereas receptor agonists attenuated allergic inflammation. These results demonstrate a protective role of the endocannabinoid system in contact allergy in the skin, and suggest a novel target for therapeutic intervention.
Keywords: Strain (Wt versus Ko) and disease state (DNFB treated versus control).
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| Overall design |
Three wildtype mice (Wt) and three Cnr1-/-/Cnr2-/- (Ko) mice were used. Contact hypersensitivity was determined always at the right ears, which therefore were treated with DNFB (Tr). Left ears of mice were kept untreated and served as control ears (C). A total of 12 hybridizations were performed (2 strains x 2 treatments X 3 biological replicates) in this experiment.
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| Contributor(s) |
Karsak M, Gaffal E, Wang-Eckhardt L, Tüting T, Zimmer A |
| Citation(s) |
17556587 |
| Submission date |
May 03, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Meliha Karsak |
| Organization name |
University of Bonn
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| Department |
Molecular Psychiatry
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| Street address |
Sigmund-Freud-Str. 25
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| City |
Bonn |
| ZIP/Postal code |
53127 |
| Country |
Germany |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM186666 |
C57BL/6J female number 1, untreated control ear (#1_Wt-C-3) |
| GSM186667 |
C57BL/6J female number 2, untreated control ear (#2_Wt-C-4) |
| GSM186668 |
C57BL/6J female number 3, untreated control ear (#3_Wt-C-7) |
| GSM186709 |
C57BL/6J female number 1, DNFB-treated allergic ear (#4_Wt-Tr-3) |
| GSM186710 |
C57BL/6J female number 2, DNFB-treated allergic ear (#5_Wt-Tr-4) |
| GSM186711 |
C57BL/6J female number 3, DNFB-treated allergic ear (#6_Wt-Tr-7) |
| GSM186743 |
Cnr1-/-/Cnr2-/- female number 1, untreated control ear (#7_Ko-C-4) |
| GSM187134 |
Cnr1-/-/Cnr2-/- female number 2, untreated control ear (#8_Ko-C-5) |
| GSM187135 |
Cnr1-/-/Cnr2-/- female number 3, untreated control ear (#9_Ko-C-7) |
| GSM187136 |
Cnr1-/-/Cnr2-/- female number 1, DNFB-treated allergic ear (#10_Ko-Tr-4) |
| GSM187137 |
Cnr1-/-/Cnr2-/- female number 2, DNFB-treated allergic ear (#11_Ko-Tr-5) |
| GSM187138 |
Cnr1-/-/Cnr2-/- female number 3, DNFB-treated allergic ear (#12_Ko-Tr-7) |
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| Relations |
| BioProject |
PRJNA99455 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7694_RAW.tar |
193.8 Mb |
(http)(custom) |
TAR (of CEL, CHP, EXP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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