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| Status |
Public on May 17, 2016 |
| Title |
Transcriptome analysis reveals global regulation in response to CO2 supplementation in oleaginous microalga Coccomyxa subellipsoidea C-169 |
| Organism |
Coccomyxa subellipsoidea C-169 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The microalga Coccomyxa subellipsoidea C-169 possesses some features that may be valuable for lipid production, and, as demonstrated in this study, can be greatly induced to produce a high amount of fatty acid by CO2 supplementation. Here we have compared the transcriptome of air group (AG, cells cultured under 0.04% CO2) and CO2-supplemented group (CG, cells cultured under 2% CO2), and found that dramatic and collaborative regulation in central metabolic pathways as well as biochemical processes occured in response to CO2 supplementation. This study gains a broad understanding of how CO2 stress regulates gene expression and eventually reveals a fine-tuned strategy adopted by C-169 to sustain rapid cell growth and lipid production, which will be helpful for the implementation of biofuels production from oleaginous microalgae.
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| Overall design |
Transcriptomic profiles of Coccomyxa subellipsoidea C-169 cultured for 4 days under two CO2 levels (0.04% and 2%, v/v) were generated by digital gene expression (DGE) analysis, in triplicate, using Illumina Hiseq2000.
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| Contributor(s) |
Peng H, Chen G, Wei D, Chen F |
| Citation(s) |
27453726 |
| |
| Submission date |
Jan 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Huifeng Peng |
| E-mail(s) |
phfterry2010@163.com
|
| Organization name |
South China University of Technology
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| Street address |
Wushan Road No.381, Tianhe District
|
| City |
Guangzhou |
| ZIP/Postal code |
510641 |
| Country |
China |
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| Platforms (1) |
| GPL21310 |
Illumina HiSeq 2000 (Coccomyxa subellipsoidea C-169) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA308212 |
| SRA |
SRP068199 |
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