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| Status |
Public on Jan 30, 2016 |
| Title |
Methylomes and Transcriptomes of Human Pluripotent-to-Cardiomyocyte Differentiation [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this report, Tompkins et al describe the derivation, differentiation stage-specific purification, and genome-wide analysis of cardiomyocytes derived from hESCs. Key features of the molecular programs that define human cardiac muscle cell differentiation were described and researchers observed that cells may harbor epigenetic DNA methylation “memories” that reflect the gene activation history of important developmental genes.
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| |
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| Overall design |
For RNA-seq. Cardiomyocyte differentiation from human embryonic stem cells (H7). 11 time point pilot time series. D3 and D4 samples FACS sorted for primitive and cardiac mesoderm isolation, respectively. Data from negatives sorts (minus) included as well.
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| |
|
| Contributor(s) |
Tompkins JD |
| Citation(s) |
26981572 |
| |
| Submission date |
Jan 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Joshua Tompkins |
| E-mail(s) |
jtompkins@coh.org
|
| Organization name |
City of Hope
|
| Department |
DMRI
|
| Street address |
1500 E Duarte Road
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE76525 |
Methylomes and Transcriptomes of Human Pluripotent-to-Cardiomyocyte Differentiation |
|
| Relations |
| BioProject |
PRJNA307622 |
| SRA |
SRP068078 |
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