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Status |
Public on Jun 01, 2004 |
Title |
CCFAlmasan_CaP1 |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The experiment design was multifold, consisting of a time course experiment on LNCaP C4-2 human prostate adenocarcinoma cells following irradiation to a dose of 10 Sv from a Cesium-137 mixed gamma and beta source, with two additional samples, one exposed to radiation in four fractions instead of all at once, and one containing the parent LNCaP cell line. PolyA+ RNA was extracted, processed and hybridized to the Affymetrix (Santa Clara, CA, www.affymetrix.com) HGU-95 (Av1-E) chip sets, and then washed, stained and scanned according to Affymetrix's protocols contained in the GeneChip(R) Expression Analysis Manual. Transcript abundance data from the scans was processed initially with Affymetrix' Microarray Suite 5(R), then by Silicon Genetics' (Redwood City, CA, www.silicongenetics.com) GeneSpring(R). Keywords = Human Keywords = Prostate Keywords = Cancer Keywords = Adenocarcinoma Keywords = Ionizing Radiation Keywords = Microarray Keywords: ordered
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Contributor(s) |
Buchsbaum JC, Gong B, Hissong JG, Ray S, Klein EA, Heston WE, Almasan A |
Citation missing |
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Submission date |
Oct 20, 2003 |
Last update date |
Jul 08, 2016 |
Contact name |
John Gilmary Hissong |
E-mail(s) |
hissonj@ccf.org
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Phone |
216-445-7105
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Fax |
216-445-6269
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URL |
http://www.lerner.ccf.org/cancerbio/almasan/
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Organization name |
Lerner Research Institute Cleveland Clinic Foundation
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Department |
Cancer Biology
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Lab |
Almasan Lab NB40
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Street address |
9500 Euclid Ave.
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City |
Cleveland |
State/province |
OH |
ZIP/Postal code |
44195 |
Country |
USA |
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Platforms (5)
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GPL91 |
[HG_U95A] Affymetrix Human Genome U95A Array |
GPL92 |
[HG_U95B] Affymetrix Human Genome U95B Array |
GPL93 |
[HG_U95C] Affymetrix Human Genome U95C Array |
GPL94 |
[HG_U95D] Affymetrix Human Genome U95D Array |
GPL95 |
[HG_U95E] Affymetrix Human Genome U95E Array |
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Samples (25)
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Relations |
BioProject |
PRJNA87713 |