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Series GSE76186 Query DataSets for GSE76186
Status Public on Apr 28, 2016
Title Sp7/Osterix is restricted to bone-forming vertebrates where it acts as a Dlx co-factor in osteoblast specification [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Sp7/Osterix is a master regulator of osteoblast specification. To identify transcripts profile in Sp7 positive osteoblast, we performed RNA-seq on primary mouse calvarial cells obtained from Sp7-GFP reporter mice at P1. By hierarchical clustering using transcriptional profiles for chondrocytes and mouse embryonic fibroblasts (MEFs) together with the osteoblast data, we identified cell-type enriched gene expression signatures in osteoblasts, chondrocytes and MEFs. In conjunction with Sp7 ChIP-seq in osteoblast, we identified putative Sp7 targets which underlie the osteoblast regulatory program.
 
Overall design RNA-seq experiments with GFP-sorted Sp7 positive osteoblasts and chondrocytes isolated from wild-type rib cartilage at P1
 
Contributor(s) Hojo H, Ohba S, He X, Lai LP, McMahon AP
Citation(s) 27134141
Submission date Dec 20, 2015
Last update date Jun 16, 2021
Contact name Hironori Hojo
E-mail(s) hojo@tetrapod.t.u-tokyo.ac.jp
Phone +81-3-5841-1427
Organization name The University of Tokyo
Department Center for Disease Biology and Integrative Medicine
Lab Clinical Biotechnology
Street address 7-3-1 Hongo
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 113-8655
Country Japan
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM1976262 Ob_rep1
GSM1976263 Ob_rep2
GSM1976264 Ob_rep3
This SubSeries is part of SuperSeries:
GSE76187 Sp7/Osterix is restricted to bone-forming vertebrates where it acts as a Dlx co-factor in osteoblast specification
Relations
BioProject PRJNA306574
SRA SRP067609

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76186_rpkm_All.txt.gz 787.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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