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| Status |
Public on Oct 04, 2016 |
| Title |
ILC progenitor CEL-seq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we identify the c-kit+ CILP population which generates all ILC subsets including NK cells, and the CD25- ILC2-restricted Sca-1+ CILP. We mapped the transcriptional changes that occur in ILC progenitor commitment identifying new regulatory factors and provide a map for early ILC differentiation. Finally, we mapped the subsequent transcriptional changes that occur in c-kit+ CILP in absence of Id2 and Tcf7, key regulators downstream of Nfil3.
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| Overall design |
100 cells of each population were purified from wild-type, Id2-/- or TCF7-/- mice. 64 samples were analysed in 2 different experiment. We purified Common innate lymphoid progenitor (CLP), a4b7-expressing CLP, common innate lymphoid progenitor (CILP), Sca1+ CILP, and Group 2 innate lymphoid cell progenitor (ILC2p).
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| Contributor(s) |
Seillet C, Mielke LA, Zalcenstein D, Su S, Gao J, Shi W, Ritchie M, Naik S, Huntington ND, Carotta S, Belz GT |
| Citation(s) |
27705792 |
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| Submission date |
Dec 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Shian Su |
| E-mail(s) |
su.s@wehi.edu.au
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| Organization name |
Walter and Eliza Hall Institute of Medical Research
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| Department |
Molecular Medicine
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| Lab |
Ritchie Lab
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| Street address |
1G Royal Parade
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| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (70)
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| Relations |
| BioProject |
PRJNA305531 |
| SRA |
SRP067183 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE75851_counts_batch1.txt.gz |
349.0 Kb |
(ftp)(http) |
TXT |
| GSE75851_counts_batch2.txt.gz |
437.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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