|
| Status |
Public on Feb 22, 2016 |
| Title |
RNA-Seq analysis of the WEHI-231-derived stable cell lines WEHI-control and WEHI-miR-148a |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
miRNAs regulate the expression of its targets genes by promoting mRNA degradation and translational repression. The goal of this study is to perform RNA-Seq in control or miR-148a-expressing WEHI-231 cell lines for the identification of miR-148a target genes.
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| |
|
| Overall design |
WEHI-control and WEHI-miR-148a cells were stimulated with anti-IgM (2 ug/ml) for 14 h. Total RNA was extracted, enriched for polyA-containing RNAs and submitted to RNA-Seq.
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| |
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| Contributor(s) |
Gonzalez-Martin A, Xiao C |
| Citation(s) |
26901150 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI089854 |
Functional Analysis of MicroRNAs in Lymphocyte Development and Immune Tolerance |
THE SCRIPPS RESEARCH INSTITUTE |
Xiao |
|
| |
| Submission date |
Dec 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Changchun Xiao |
| E-mail(s) |
cxiao@scripps.edu
|
| Phone |
(858) 784-7640
|
| Organization name |
The Scripps Research Institute
|
| Department |
Immunology & Microbial Science
|
| Lab |
Changchun Xiao Lab
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (8)
|
| GSM1968244 |
WEHI-miR-148a, stimulated, AGM4 |
| GSM1968245 |
WEHI-control, non-stimulated, AGM1_2 |
| GSM1968246 |
WEHI-miR-148a, non-stimulated, AGM2_2 |
| GSM1968247 |
WEHI-control, stimulated, AGM3_2 |
| GSM1968248 |
WEHI-miR-148a, stimulated, AGM4_2 |
|
| Relations |
| BioProject |
PRJNA305410 |
| SRA |
SRP067133 |
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