|
| Status |
Public on Feb 20, 2017 |
| Title |
miRNA expression data from neonatal rat ventricular myocytes (NRVM) silenced for KChIP2 |
| Platform organism |
synthetic construct |
| Sample organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Loss of KChIP2 during cardiac stress has been suggested to have a transcriptional impact on cardiac ion channels through altered miRNA activity, contributing to maladaptive electrical remodeling. Therefore, we tested the consequence of KChIP2 loss, in the absence of cardiac stress, by treating cultured neonatal rat ventricular myocytes with siRNA for KChIP2 and subsequently performed miRNA microarray analysis to identify up-regulation of potential miRNA targets.
|
| |
|
| Overall design |
NRVMs were isolated from the hearts of neonatal (day 1-2) Sprague Dawley rats. They were subsequently plated and cultured for 24-36 hrs then treated with either a control or KChIP2 siRNA. Cells were left in culture for an additional 72 hrs following siRNA delivery, after which they were collected for total RNA.
|
| |
|
| Citation(s) |
28263709 |
| |
| Submission date |
Dec 08, 2015 |
| Last update date |
Jun 28, 2017 |
| Contact name |
Isabelle Deschenes |
| E-mail(s) |
isabelle.deschenes@case.edu
|
| Organization name |
Case Western Reserve University
|
| Street address |
2500 Metrohealth Drive
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA305405 |
