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Series GSE75806 Query DataSets for GSE75806
Status Public on Feb 20, 2017
Title miRNA expression data from neonatal rat ventricular myocytes (NRVM) silenced for KChIP2
Platform organism synthetic construct
Sample organism Rattus norvegicus
Experiment type Non-coding RNA profiling by array
Summary Loss of KChIP2 during cardiac stress has been suggested to have a transcriptional impact on cardiac ion channels through altered miRNA activity, contributing to maladaptive electrical remodeling. Therefore, we tested the consequence of KChIP2 loss, in the absence of cardiac stress, by treating cultured neonatal rat ventricular myocytes with siRNA for KChIP2 and subsequently performed miRNA microarray analysis to identify up-regulation of potential miRNA targets.
Overall design NRVMs were isolated from the hearts of neonatal (day 1-2) Sprague Dawley rats. They were subsequently plated and cultured for 24-36 hrs then treated with either a control or KChIP2 siRNA. Cells were left in culture for an additional 72 hrs following siRNA delivery, after which they were collected for total RNA.
Citation(s) 28263709
Submission date Dec 08, 2015
Last update date Jun 28, 2017
Contact name Isabelle Deschenes
Organization name Case Western Reserve University
Street address 2500 Metrohealth Drive
City Cleveland
State/province OH
ZIP/Postal code 44109
Country USA
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (2)
GSM1968161 NRVM control siRNA treated
GSM1968162 NRVM KChIP2 siRNA treated
BioProject PRJNA305405

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75806_RAW.tar 1.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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