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| Status |
Public on May 31, 2010 |
| Title |
Toxicogenomic Effect of Burkholderia pseudomallei in a Human Macrophage Cell (THP-1) Model of Acute Melioidosis Using Whole-genome Microarray |
| Platform organism |
Homo sapiens |
| Sample organisms |
Homo sapiens; Burkholderia pseudomallei |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiles of human cell (THP-1) lines exposed to a novel Daboiatoxin (DbTx) isolated from Daboia russelli russelli, and specific cytokines and inflammatory pathways involved in acute infection caused by Burkholderia pseudomallei.
Keywords: Melioidosis, Burkholderia pseudomallei, Daboiatoxin, Cytokines, Inflammation.
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| Overall design |
1. Group I:- Human monocytic macrophage (THP-1) cell lines grown in the culture medium without any bacterial infection served as untreated control group (Three Biological Replicates).
2. Group II:- THP-1 cells were infected with Burkholderia pseudomallei (A600 nm = OD 0.6, ~5 x 107 cfu/ml) for 24h served as a disease control group (Three Biological Replicates).
3. Group III:- THP-1 cells were infected with B. pseudomallei and treated with Daboiatoxin (0.5 mM) isolated from Daboia russelli russelli venom served as a treatment group (Three Biological Replicates).
4. Group IV:- THP-1 cells were infected with B. pseudomallei (A600 nm = OD 0.6, ~5 x 107 cfu/ml) treated with standard antimicrobial drug ceftazidime (10mg/ml) served as a drug control (Three Biological Replicates).
5. Group V:- THP-1 cells were exposed to Daboiatoxin (0.5 mM) without bacterial infection (Three Biological Replicates).
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| Contributor(s) |
Arjunan P, Ramar PS, Ponnampalam G, Lay TA, Ooi EE |
| Citation(s) |
26592245 |
| Submission date |
Apr 23, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Pachiappan Arjunan |
| Organization name |
NUS, NIH, GRU
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| Department |
Anatomy, Unit on Retinal Vascular Neurobiology, BMB
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| Lab |
VTRP, URVN, BMB
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| Street address |
1120 15th ST, Laney walker Blvd,
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| City |
Augusta |
| State/province |
Georgia |
| ZIP/Postal code |
30904 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (15)
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| GSM183520 |
Human monocytic macrophage (THP-1) cell lines served as untreated control (Replicate 1) |
| GSM183521 |
Human monocytic macrophage (THP-1) cell lines served as untreated control (Replicate 2) |
| GSM183522 |
Human monocytic macrophage (THP-1) cell lines served as untreated control (Replicate 3) |
| GSM183523 |
G-(II)_THP-1+B. pseudomallei 24h_Rep1. |
| GSM183525 |
G-(II)_THP-1+B. pseudomallei 24h_Rep2. |
| GSM183527 |
G-(II)_THP-1+B. pseudomallei 24h_Rep3. |
| GSM183530 |
G-(III)_THP-1+B. pseudomallei 24h+DbTx_Rep1. |
| GSM183532 |
G-(III)_THP-1+B. pseudomallei 24h+DbTx_Rep2. |
| GSM183533 |
G-(III)_THP-1+B. pseudomallei 24h+DbTx_Rep3. |
| GSM183534 |
G-IV_THP-1+B. pseudomallei 24h+CAZ_Rep1. |
| GSM183535 |
G-IV_THP-1+B. pseudomallei 24h+CAZ_Rep2. |
| GSM183536 |
G-IV_THP-1+B. pseudomallei 24h+CAZ_Rep3. |
| GSM183537 |
G-V_THP-1+DbTx_Rep1. |
| GSM183538 |
G-V_THP-1+DbTx_Rep2. |
| GSM183539 |
THP-1+ treated with DbTx_Rep3. |
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| Relations |
| BioProject |
PRJNA100355 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7577_RAW.tar |
43.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
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