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| Status |
Public on Dec 18, 2015 |
| Title |
Loss of motoneuron-specific microRNA-218 causes systemic neuromuscular failure [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We investigated microRNA expression in motoneurons by performing small RNA sequencing of fluorescence-activated cell sorting (FACS)-isolated motoneurons labelled with the Hb9:gfp transgenic reporter and Hb9:gfp negative non-motoneurons including spinal interneurons. We find that one microRNA, microRNA-218, is highly enriched and abundantly expressed in motoneurons. Furthermore, we find that miR-218 is transcribed from alternative, motoneuron-specific alternative promoters embedded within the Slit2 and Slit3 genes by performing RNA sequencing of FACS-isolated motoneurons and a dissected embryonic floor plate cells which served as a control. Next, we performed RNA sequencing of FACS-isolated wild type (WT) motoneurons and motoneurons lacking miR-218 expression (218DKO motoneurons), and find that a large set of genes (named 'TARGET218' genes) with predicted miR-218 binding sites are de-repressed in the absence of miR-218 expression. Finally, we examine the expression of TARGET218 genes in other neuronal subpopulations by FACS-isolating V1, V2a, and V3 interneurons expressing Cre-inducible fluorescent reporters and performing RNA sequencing. We find that the TARGET218 network of genes is depleted in wild-type motoneurons versus these interneuron types. Additionally, these genes are expressed at similar levels in 218DKO motoneurons compared with interneuron subtypes, suggesting that this genetic network.
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| Overall design |
Examination of mRNA expression in spinal progenitor, glial, and neuronal subpopulations.
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| Contributor(s) |
Amin ND, Pfaff SL |
| Citation(s) |
26680198 |
| |
| Submission date |
Dec 01, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Neal D Amin |
| E-mail(s) |
namin@salk.edu
|
| Organization name |
Salk Institute for Biological Studies
|
| Department |
Gene Expression Laboratory
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| Lab |
Pfaff
|
| Street address |
10010 N Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE75601 |
Loss of motoneuron-specific microRNA-218 causes systemic neuromuscular failure |
|
| Relations |
| BioProject |
PRJNA304986 |
| SRA |
SRP066961 |
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