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| Status |
Public on Apr 05, 2016 |
| Title |
Determination of a comprehensive alternative splicing regulatory network and the combinatorial regulation by key factors during Epithelial-to-Mesenchymal Transition [EMT.time course] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The epithelial to mesenchymal transition (EMT) is an essential biological process during embryonic development and has also been implicated in cancer metastasis. Previous studies have characterized transcriptional regulation and key transcription factors that impact EMT. However, the role of alternative splicing (AS) regulation in EMT has only recently emerged and remains relatively uncharacterized. Here we used a robust in vitro EMT model to dynamically and comprehensively characterize splicing switches during EMT in a temporal manner.
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| Overall design |
We generated a H358 clone stably expressing a doxycycline (Dox)-inducible cDNA encoding a Zeb1-mCherry fusion protein. Over a 7-day time course following Dox treatment, cells have undergone EMT. We harvested total RNA and protein at each day of the EMT time course and a no Dox-treated control in biological triplicates. We made cDNA libraries for each replicate and subjected them to RNA-seq.
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| Contributor(s) |
Xing Y, Carstens R |
| Citation(s) |
27044866 |
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| Submission date |
Nov 30, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Yi Xing |
| Organization name |
UCLA
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| Department |
Department of Microbiology, Immunology, & Molecular Genetics
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| Street address |
650 Charles E. Young Dr. South
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-7278 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (24)
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| GSM1956977 |
Day1, rep1 (Paired-end) |
| GSM1956978 |
Day1, rep2 (Paired-end) |
| GSM1956979 |
Day1, rep3 (Paired-end) |
| GSM1956980 |
Day2, rep1 (Paired-end) |
| GSM1956981 |
Day2, rep2 (Paired-end) |
| GSM1956982 |
Day2, rep3 (Paired-end) |
| GSM1956983 |
Day3, rep1 (Paired-end) |
| GSM1956984 |
Day3, rep2 (Paired-end) |
| GSM1956985 |
Day3, rep3 (Paired-end) |
| GSM1956986 |
Day4, rep1 (Paired-end) |
| GSM1956987 |
Day4, rep2 (Paired-end) |
| GSM1956988 |
Day4, rep3 (Paired-end) |
| GSM1956989 |
Day5, rep1 (Paired-end) |
| GSM1956990 |
Day5, rep2 (Paired-end) |
| GSM1956991 |
Day5, rep3 (Paired-end) |
| GSM1956992 |
Day6, rep1 (Paired-end) |
| GSM1956993 |
Day6, rep2 (Paired-end) |
| GSM1956994 |
Day6, rep3 (Paired-end) |
| GSM1956995 |
Day7, rep1 (Paired-end) |
| GSM1956996 |
Day7, rep2 (Paired-end) |
| GSM1956997 |
Day7, rep3 (Paired-end) |
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| This SubSeries is part of SuperSeries: |
| GSE75492 |
Determination of a comprehensive alternative splicing regulatory network and the combinatorial regulation by key factors during Epithelial-to-Mesenchymal Transition |
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| Relations |
| BioProject |
PRJNA304414 |
| SRA |
SRP066794 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE75487_FPKMs.EMT.timeCourse.xlsx.gz |
6.5 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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