|
| Status |
Public on Nov 30, 2015 |
| Title |
RNA splicing alteration on glioblastoma and normal neural stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified PHF5A as a functional synthetic-lethal hit in glioblastoma stem cells compared to normal neural stem cells. We wanted to perform analysis of RNA isoforms present in glioblastoma or normal neural stem cells with or without PHF5A depletion. We performed shRNA knockdown of PHF5A or used non-silencing shRNA as a control, selected infected cells with puromycin, and isolated RNA for sequencing.
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| |
|
| Overall design |
We analyzed RNA from either normal neural stem cells or two different glioblastoma specimens aster either control knockdown, or two different shRNA sequences against the PHF5A gene transcript.
|
| |
|
| Contributor(s) |
Paddison P, Bradley R, Hubert C |
| Citation(s) |
23651857 |
| |
| Submission date |
Nov 18, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Christopher G. Hubert |
| E-mail(s) |
hubertc@ccf.org
|
| Phone |
2166361008
|
| Organization name |
Cleveland Clinic
|
| Department |
Stem Cell Biology and Regenerative Medicine
|
| Street address |
9500 Euclid Ave.
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA302647 |
| SRA |
SRP066371 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE75147_regulated.phf5a_kd_402.down.CB660.list.txt.gz |
10.2 Kb |
(ftp)(http) |
TXT |
| GSE75147_regulated.phf5a_kd_402.down.G166.list.txt.gz |
97.6 Kb |
(ftp)(http) |
TXT |
| GSE75147_regulated.phf5a_kd_402.up.CB660.list.txt.gz |
6.7 Kb |
(ftp)(http) |
TXT |
| GSE75147_regulated.phf5a_kd_402.up.G166.list.txt.gz |
33.5 Kb |
(ftp)(http) |
TXT |
| GSE75147_regulated.phf5a_kd_861.down.0827.list.txt.gz |
154.4 Kb |
(ftp)(http) |
TXT |
| GSE75147_regulated.phf5a_kd_861.down.G166.list.txt.gz |
323.4 Kb |
(ftp)(http) |
TXT |
| GSE75147_regulated.phf5a_kd_861.up.0827.list.txt.gz |
35.0 Kb |
(ftp)(http) |
TXT |
| GSE75147_regulated.phf5a_kd_861.up.G166.list.txt.gz |
57.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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