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| Status |
Public on Jul 22, 2020 |
| Title |
Role of the histone demethylase Kdm6b/Jmjd3 in somatic cell reprogramming [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Somatic cells can be reprogrammed to pluripotent stem cells through the addition of just four transcription factors, OCT4, SOX2, KLF4 and c-MYC (OSKM). Although OSKM initiates reprogramming it is clear that extensive epigenetic remodeling is required to complete reprogramming. Critically, OSKM do not directly activate gene expression but instead recruit co-activators and co-repressors that remodel the local chromatin and in some way make the cells permissive for reprogramming. Consequently understanding how epigenetic co-repressors and co-activators are involved in reprogramming is a critical step in understanding the reprogramming process in detail. In this study we explored the role of the lysine-specific demethylase Kdm6b/Jmjd3 and its role in the reprogramming of somatic cells to pluripotent cells.
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| Overall design |
RNA-seq data consists of MEFs at passage 2 or 4, with or withour JMJD3 overexpression, and reprogrammed cells with the ectopic expression of OSKM (Oct4, Sox2, Klf4, c-Myc), along with either control transfections (FLAG, shLuc) or JMJD3 overexpression or Jmjd3 or Utx knockdown. Two time points in the reprogramming time course were profiled: Day 4 (early) and Day 8 (late)
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| Contributor(s) |
Hutchins AP, Qin B, Yinghua H |
| Citation missing |
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| |
| Submission date |
Nov 13, 2015 |
| Last update date |
Jul 23, 2020 |
| Contact name |
Andrew P Hutchins |
| E-mail(s) |
andrewh@sustech.edu.cn
|
| Organization name |
Southern University of Science and Technology
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| Department |
Bioinformatics
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| Lab |
Bioinformatics and Genomics
|
| Street address |
1088 Xueyuan Rd
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| City |
Shenzhen |
| ZIP/Postal code |
518055 |
| Country |
China |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (29)
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| This SubSeries is part of SuperSeries: |
| GSE75005 |
Role of the histone demethylase Kdm6b/Jmjd3 in somatic cell reprogramming |
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| Relations |
| BioProject |
PRJNA302172 |
| SRA |
SRP066186 |
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