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| Status |
Public on Jul 07, 2016 |
| Title |
Single Cell Expression Profiling Resolves the Transcriptional Programs of Early Mesoderm Diversification |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We analyzed 1,205 cells from the epiblast and nascent mesoderm of gastrulating mouse embryos by single cell RNA-seq, representing the first transcriptome-wide in vivo view of mammalian gastrulation. Based on the distribution of key marker genes and their spatiotemporal expression patterns within the embryo, we identified 10 cell clusters corresponding to the epiblast and developing mesodermal populations. This enabled us to explore the global gene expression dynamics associated with the induction of Brachyury, anterior-posterior patterning of the primitive streak and the earliest stages of blood development.
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| Overall design |
Examination of Gata1 binding in Runx1-ires-GFP+/Gata1-mCherry+ (primitive erythroid) cells derived from 5 days of differentiation of ESCs towards haematopoietic cells
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| Contributor(s) |
Scialdone A, Tanaka Y, Jawaid W, Moignard V, Wilson NK, Macaulay IC, Marioni JC, Göttgens B |
| Citation(s) |
27383781 |
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| Submission date |
Nov 13, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Evangelia Diamanti |
| E-mail(s) |
ed347@cam.ac.uk
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| Phone |
01223 62317
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| Organization name |
University of Cambridge
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| Department |
Haematology
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| Lab |
Gottgens
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| Street address |
Wellcome Trust / MRC Building, Hills Road
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA302149 |
| SRA |
SRP066168 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE74994_RAW.tar |
683.9 Mb |
(http)(custom) |
TAR (of BED, BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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