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Series GSE74552 Query DataSets for GSE74552
Status Public on Oct 30, 2016
Title Transcriptional profiling of post-positive selection thymocytes from HOIL- and SHARPIN-deficient mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mice lacking components of the linear ubiquitin chain assembly complex (LUBAC) exhibit defects in thymocyte differentiation. To determine how two LUBAC components, HOIL and SHARPIN, mediate their effects, we purified thymocytes from CD4Cre Hoil-fl/fl or Sharpin-cpdm mice and compared their transcriptional profile to WT controls. We focused on the CD69+ MHCI low and CD69+ MHCI high subsets that follow positive selection.
Overall design Thymocytes were prepared from 3 wildtype mice, 3 Cd4Cre Hoil-floxed mice and 3 Sharpin-cpdm mutant mice. CD69+ MHC I high and CD69+ MHC I low cells from each mouse were FACS purified on a MoFlo cell sorter.
Contributor(s) Gray DH, Hu Y, Smyth GK
Citation(s) 27857075
Submission date Oct 30, 2015
Last update date May 15, 2019
Contact name Gordon K Smyth
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM1922755 Hoil 1 M1hi
GSM1922756 Hoil 1 M1lo
GSM1922757 Hoil 2 M1hi
BioProject PRJNA300687
SRA SRP065560

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE74552_HoilSharpin-GenewiseCounts.txt.gz 620.1 Kb (ftp)(http) TXT
GSE74552_RAW.tar 3.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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