|
| Status |
Public on Oct 30, 2016 |
| Title |
Transcriptional profiling of post-positive selection thymocytes from HOIL- and SHARPIN-deficient mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mice lacking components of the linear ubiquitin chain assembly complex (LUBAC) exhibit defects in thymocyte differentiation. To determine how two LUBAC components, HOIL and SHARPIN, mediate their effects, we purified thymocytes from CD4Cre Hoil-fl/fl or Sharpin-cpdm mice and compared their transcriptional profile to WT controls. We focused on the CD69+ MHCI low and CD69+ MHCI high subsets that follow positive selection.
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| Overall design |
Thymocytes were prepared from 3 wildtype mice, 3 Cd4Cre Hoil-floxed mice and 3 Sharpin-cpdm mutant mice. CD69+ MHC I high and CD69+ MHC I low cells from each mouse were FACS purified on a MoFlo cell sorter.
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| |
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| Contributor(s) |
Gray DH, Hu Y, Smyth GK |
| Citation(s) |
27857075 |
| |
| Submission date |
Oct 30, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA300687 |
| SRA |
SRP065560 |
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