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Series GSE74184 Query DataSets for GSE74184
Status Public on Nov 12, 2015
Title Methylome analysis of deoxyadenosines in higher eukaryotes
Organisms Escherichia coli; Xenopus laevis; Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Here, we report that we detected N-6-methyl-deoxyadenosine (dA6m) not only in frog DNA, but also in other species including mouse and humans. Our methylome analysis revealed that dA6m is widely distributed across the eukaryotic genome, and it is present in different cell types. dA6m is commonly depleted in gene exons, whilst it remains abundant in introns. Finally, we have identified the putative consensus sequence, where there is preferential methylation. Since dA6m is a direct modification of DNA, it has the potential to generate a significant impact on different biological areas. Ultimately, our work shows that deoxycytidine modifications might not be the only ones in higher eukaryotes, suggesting that DNA alterations might be more widespread than previously thought.
Overall design Determining regions of deoxyadenosine methylation in M. musculus kidney and X. laevis fat, oviduct and testes
Contributor(s) Koziol MJ, Bradshaw CR, Allen GE, Costa AS, Frezza C, Gurdon JB
Citation(s) 26689968
Submission date Oct 20, 2015
Last update date May 15, 2019
Contact name Charles Bradshaw
Organization name University of Cambridge
Department Gurdon Institute
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
Platforms (4)
GPL18133 Illumina HiSeq 2500 (Escherichia coli)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL18936 Illumina HiSeq 2500 (Xenopus laevis)
Samples (28)
GSM1912879 E. coli Dam- input
GSM1912880 E. coli Dam- IP
GSM1912881 E. coli Dam+ input
BioProject PRJNA299278
SRA SRP065025

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Supplementary file Size Download File type/resource
GSE74184_RAW.tar 2.0 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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