|
| Status |
Public on Apr 24, 2017 |
| Title |
Transcriptomic analysis of Mtb H37Rrv and MtbΔwhiB4 upon treatment with 0.25 mM CHP |
| Organism |
Mycobacterium tuberculosis H37Rv |
| Experiment type |
Expression profiling by array
|
| Summary |
WhiB4 regulates expression of anti-oxidant genes and its own expression was shown to be down-regulated upon exposure to oxidatants like CHP. Further MtbΔwhiB4 was shown to survive better under oxidaitve stress. Thus, we did microarray upon exposure to 0.25 mM CHP to study comprehensively the WhiB4 regulon under oxidative stress.
|
| |
|
| Overall design |
Mtb H37Rv and MtbΔwhiB4 were grown till early log phase and exposed to 0.25 mM CHP for 2 hours at 37 degree at 150rpm. Four and two independent experiments were done for each of the strain, respectively.
|
| |
|
| Contributor(s) |
Parikh P, Chawla M, Singh A, Mehta M |
| Citation(s) |
30149290 |
| |
| Submission date |
Oct 09, 2015 |
| Last update date |
Sep 06, 2018 |
| Contact name |
Amit Singh |
| E-mail(s) |
asingh@mcbl.iisc.ernet.in
|
| Organization name |
Indian Institute of Science
|
| Department |
MCBL
|
| Street address |
C V Raman Avenue
|
| City |
Bangalore |
| ZIP/Postal code |
560012 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL21002 |
Agilent-034585 UDSC M. tuberculosis H37Rv Validated Array (PROBE ID version) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA298294 |
Less...
More...