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Series GSE7362

Query DataSets for GSE7362

Status

Public on Dec 23, 2007

Title

The contribution of different nutrients to spore germination in Saccharomyces cerevisiae

Organism

Saccharomyces cerevisiae

Experiment type

Expression profiling by array

Summary

To study the signals and pathways underlying spore germination we examined the global changes in gene expression during this process.
We find that the germination process can be divided into two distinct stages. During the first stage, the induced spores respond only to glucose. The transcription program during this stage recapitulates the general transcription response of yeast cells to glucose. Only during the second phase are the cells able to sense and respond to other nutritional components in the environment. Components of the mitotic machinery are involved in spore germination but in a distinct pattern. In contrast to the mitotic cell cycle, growth related events during germination are not coordinated with nuclear events and are separately regulated.
Genome-wide expression profiling enables us to follow the progression of spore germination, thus dividing this process into two major stages and to identify germination-specific regulation of components of the mitotic cell cycle machinery.
Keywords: Time course

Overall design

Wild type SK1 cells (DS1) were grown to saturation in YPD (2% yeast extract, 4% bactopeptone, 4% glucose) at 30 C for 24 hours, washed in sterile water and plated on sporulation plates (0.25% yeast extract, 1.5% Potassium acetate, 0.05% glucose supplemented with all amino acids). 3 days old asci were harvested and suspended in either 2% glucose or synthetic minimal medium without glucose (0.17% yeast nitrogen base,0.5% ammonium sulfate, supplemented with amino acids). RNA for microarray hybridization was extracted at the indicated times (23 samples). Following two hours of incubation part of the culture was transferred to rich medium (YPD) to allow spore germination and RNA for microarray hybridization was extracted at the indicated times (15 samples).

Contributor(s)

Joseph-Strauss D, Barkai N, Simchen G

Citation(s)

Submission date

Mar 24, 2007

Last update date

Mar 17, 2012

Contact name

Naama Barkai

E-mail(s)

naama.barkai@weizmann.ac.il, simchen@vms.huji.ac.il

Organization name

The Weizmann Institute of Science

Department

Molecular Genetics

Lab

Naama Barkai

Street address

P.O box 26

City

Rehovot

ZIP/Postal code

76100

Country

Israel

Platforms (1)

UHN Yeast 6.4kv6

Samples (38)

More...

GSM177221	Spores_incubation in different nutrients_SPO
GSM177222	Spores_incubation in glucose_15 minutes
GSM177223	Spores_incubation in glucose_30 minutes

GSM177224	Spores_incubation in glucose_45 minutes
GSM177225	Spores_incubation in glucose_1 hour
GSM177226	Spores_incubation in glucose_1 hour and 30 minutes
GSM177227	Spores_incubation in glucose_2 hours
GSM177228	Spores_incubation in glucose_2 hours and 30 minutes
GSM177229	Spores_incubation in glucose_3 hours
GSM177230	Spores_incubation in glucose_3 hours and 30 minutes
GSM177231	Spores_incubation in glucose_4 hours
GSM177232	Spores_incubation in glucose_6 hours
GSM177233	Spores_incubation in nitrogen_15 minutes
GSM177234	Spores_incubation in nitrogen_30 minutes
GSM177235	Spores_incubation in nitrogen_45 minutes
GSM177236	Spores_incubation in nitrogen_1 hour
GSM177237	Spores_incubation in nitrogen_1 hour and 30 minutes
GSM177238	Spores_incubation in nitrogen_2 hours
GSM177239	Spores_incubation in nitrogen_2 hours and 30 minutes
GSM177240	Spores_incubation in nitrogen_3 hours
GSM177241	Spores_incubation in nitrogen_3 hours and 30 minutes
GSM177242	Spores_incubation in nitrogen_4 hours and 30 minutes
GSM177243	Spores_incubation in nitrogen_6 hours
GSM177244	Spores_germination following 2 hours of pre-incubation in glucose_30 minutes
GSM177245	Spores_germination following 2 hours of pre-incubation in glucose_1 hour
GSM177246	Spores_germination following 2 hours of pre-incubation in glucose_1 hour and 30 minutes
GSM177247	Spores_germination following 2 hours of pre-incubation in glucose_2 hours
GSM177248	Spores_germination following 2 hours of pre-incubation in glucose_2 hours and 30 minutes
GSM177249	Spores_germination following 2 hours of pre-incubation in glucose_3 hours
GSM177250	Spores_germination following 2 hours of pre-incubation in glucose_3 hours and 30 minutes
GSM177251	Spores_germination following 2 hours of pre-incubation in glucose_4 hours
GSM177252	Spores_germination following 2 hours of pre-incubation in nitrogen_30 minutes
GSM177253	Spores_germination following 2 hours of pre-incubation in nitrogen_1 hour
GSM177254	Spores_germination following 2 hours of pre-incubation in nitrogen_1 hour and 30 minutes
GSM177255	Spores_germination following 2 hours of pre-incubation in nitrogen_2 hours
GSM177256	Spores_germination following 2 hours of pre-incubation in nitrogen_2 hours and 30 minutes
GSM177257	Spores_germination following 2 hours of pre-incubation in nitrogen_3 hours and 30 minutes
GSM177258	Spores_germination following 2 hours of pre-incubation in nitrogen_4 hours

This SubSeries is part of SuperSeries:

Spore germination in Saccharomyces cerevisiae

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE7362_RAW.tar	65.1 Mb	(http)(custom)	TAR (of SRR)

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