|
| Status |
Public on Dec 14, 2015 |
| Title |
Gene expression analysis to identify Klf2 target genes in B-cell progenitors [Klf2_KO] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The sequential activation of distinct developmental gene networks governs the ultimate identity of a cell, but the mechanisms by which downstream programs are activated are incompletely understood. The preB-cell receptor (preBCR) is an important checkpoint of B-cell development and essential for a preB-cell to traverse into an immature B-cell. Here, we show that activation of Mef2 transcription factors by preBCR is necessary for initiating the subsequent genetic network. We demonstrate that B-cell development is blocked at the preB-cell stage in mice deficient for Mef2c and Mef2d transcription factors and that preBCR signaling enhances the transcriptional activity of Mef2c/d through phosphorylation by the ERK5 mitogen activating kinase. This activation is instrumental in inducing Krüppel-like factor 2 and several immediate early genes of the AP1 and Egr family. Finally, we show that Mef2 proteins cooperate with the products of their target genes (Irf4 and Egr2) to induce secondary waves of transcriptional regulation. Our findings uncover a novel role for Mef2c/d in coordinating the transcriptional network that promotes early B-cell development.
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| |
|
| Overall design |
RNA-seq experiments were performed from Klf2 knockout proB-cells versus control cells to identify genes regulated by Klf2
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| |
|
| Contributor(s) |
Herglotz J, Indenbirken D, Stocking C |
| Citation(s) |
26660426 |
| |
| Submission date |
Sep 29, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Julia Herglotz |
| E-mail(s) |
julia.preukschas@hpi.uni-hamburg.de
|
| Organization name |
Heinrich-Pette-Institut
|
| Street address |
Martinistr. 52
|
| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (4)
|
| GSM1897972 |
RNA-seq analysis of proB-cell from Mb1Cre mice_L001 |
| GSM1897973 |
RNA-seq analysis of proB-cell from Mb1Cre mice_L002 |
| GSM1897974 |
RNA-seq analysis of proB-cell from Klf2 Mb1Cre mice_L001 |
| GSM1897975 |
RNA-seq analysis of proB-cell from Klf2 Mb1Cre mice_L002 |
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| This SubSeries is part of SuperSeries: |
| GSE73563 |
Essential control of early B-cell development by Mef2 transcription factors |
|
| Relations |
| BioProject |
PRJNA297343 |
| SRA |
SRP064312 |
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