|
| Status |
Public on Sep 15, 2016 |
| Title |
Global gene expression profile of primary mouse brain and glioblastoma tissues |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mouse glioblastomas were induced by lentiviral vector expressing HrasG12V and shRNA against p53. Tumor tissues were isolated from mice reached clinical endpoints. RNA was isolated using the RNeasy kit according to manufacturer’s protocol with the addition of DNase (Qiagen). cDNA libraries were prepared using the TruSeq RNA Sample Prep kit (Illumina). RNA sequencing was performed using a HiSeq 2500 Sequencing System (Illumina).
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| |
|
| Overall design |
3 normal mouse brain samples compared to 5 glioblastoma samples by standard RNAseq method.
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| |
|
| Contributor(s) |
Xia Y, Myskiw C |
| Citation(s) |
26824076 |
| |
| Submission date |
Sep 17, 2015 |
| Last update date |
Mar 22, 2021 |
| Contact name |
Yifeng Xia |
| E-mail(s) |
yxia@salk.edu
|
| Phone |
858-453-4100
|
| Organization name |
Salk Insitute
|
| Department |
Laboratory of Genetics
|
| Lab |
Verma
|
| Street address |
10010 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA296067 |
| SRA |
SRP063872 |
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