|
| Status |
Public on Apr 01, 2016 |
| Title |
Determination of the Sp5 regulated transcriptome in differentiating ES cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sp5 is a target and critical transducer of the Wnt/beta-catenin signaling pathway and is essential for mesoderm differentiation in vertebrate embryos. We established a doxycycline inducible ES cell line to over-express Flag epitope-tagged Sp5 protein in differentiating ES cells which have the capacity to form mesoderm cells in vitro. The goal of this study was to identify the genes/gene networks regulated by Sp5 to understand how it regulates mesoderm differentiation by identifying important target genes which may mediate its activity.
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| Overall design |
Total RNA was extracted from ES cells after 3 days of differentiating following F-Sp5 over-expression from day 2 to 3. We compared the transcriptome of differentially expressed genes between untreated (minus) and doxycycline treated (plus) ES cells to identify Sp5 regulated genes.
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| |
|
| Contributor(s) |
Yamaguchi TP, Kennedy MW |
| Citation(s) |
26969725 |
| |
| Submission date |
Sep 16, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Mark W Kennedy |
| E-mail(s) |
mark.kennedy@nih.gov
|
| Organization name |
NIH/NCI
|
| Department |
Cancer and Developmental Biology
|
| Lab |
Cell Signaling in Vertebrate Development
|
| Street address |
1050 Boyles St., Bldg 539, Rm 205
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE73084 |
Sp5 and Sp8 recruit b-catenin to the Tcf1-Lef1 enhanceosome to activate Wnt target gene transcription |
|
| Relations |
| BioProject |
PRJNA296126 |
| SRA |
SRP063827 |
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