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| Status |
Public on Dec 16, 2016 |
| Title |
Targeting metastasis stem cells through the fatty acid receptor CD36 (I) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Metastasis is the leading cause of cancer-related deaths. For most human cancers, the identity of the cells that initiate and promote metastasis is still unknown, hampering our ability to develop therapies to prevent or inhibit the spread of tumour cells to distant sites. Using an orthotopic model of human oral squamous cell carcinoma (OSCC), we have now identified a subpopulation of CD44bright cells within the primary lesion with the highest potential to develop lymph node and lung metastasis. This population is slow-cycling, expresses high levels of the receptor CD36 at the cell membrane and relies on fatty acid metabolism to thrive in lymph nodes and bronchoalveolar environments. Importantly, inhibition of CD36 by either shRNA or neutralizing monoclonal antibodies severely impairs metastatic spread of primary OSCC patient samples and established cell lines. Further underscoring its importance, CD36 overexpression in poorly disseminating tumours confers an aggressive metastatic behavior. Analyses of public gene expression data indicate that the presence of the signature-defining CD36+ cells also strongly correlates with a poor prognosis in patients with lung SCC, ovarian cancer, bladder cancer, or luminal breast cancer. By identifying metastasis-promoting cells and then targeting them with CD36 inhibition, novel anti-metastatic therapies could be developed for patients with these types of tumours.
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| Overall design |
4 biological replicates from SCC-25 cell line were included (set 8A, set 8B, set 9B and set 10A). From each biological replicate, 3 independent cell populations were sorted DID+,DID- and CD44. From set 8B, an additional technical replicate was done.
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| Contributor(s) |
Aznar-Benitah S, Mejetta S, Stephan-Otto Attolini C |
| Citation(s) |
27974793 |
| Submission date |
Sep 11, 2015 |
| Last update date |
Mar 27, 2018 |
| Contact name |
Antoni Berenguer-LLergo |
| E-mail(s) |
aberenguerl@tauli.cat
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| Organization name |
Parc Taulí Research and Innovation Institute (I3PT)
|
| Department |
A8G2 Group - Rheumatology Service
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| Street address |
Parc del Taulí, 1
|
| City |
Sabadell |
| State/province |
Barcelona |
| ZIP/Postal code |
08208 |
| Country |
Spain |
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| Platforms (1) |
| GPL16699 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE72939 |
Targeting metastasis stem cells through the fatty acid receptor CD36 |
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| Relations |
| BioProject |
PRJNA296106 |
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