|
| Status |
Public on Jun 17, 2016 |
| Title |
Histone arginine methyltransferase CARM1 Is Crucial for Epigenetic Regulation of Starvation-induced Autophagy |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Autophagy is a highly conserved self-digestion process, essential to maintain homeostasis and viability in response to nutrient starvation. Although the components of autophagy in the cytoplasm have been well-studied, molecular basis for the epigenetic regulation of autophagy is poorly understood. Here, we identify histone arginine methyltransferase CARM1 as a critical component of autophagy. We found that nutrient starvation increased CARM1 protein level and subsequently histone H3R17 dimethylation. Genome-wide analyses reveal that CARM1 exerts transcriptional coactivator function on autophagy-related genes and lysosomal genes through TFEB. Our findings demonstrate a previously unrecognized role of CARM1-dependent histone arginine methylation as a critical nuclear event of autophagy.
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| |
|
| Overall design |
1) Gene expression profiling of WT and Carm1 KO MEFs under nutrient rich condition or glucose starvation. 2) Examination of histone H3R17 dimethylation in WT MEFs upon glucose starvation
|
| |
|
| Contributor(s) |
Shin HR, Won K, Baek S |
| Citation(s) |
27309807 |
| |
| Submission date |
Sep 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Kyoung Jae Won |
| E-mail(s) |
wonk@mail.med.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Genetics
|
| Lab |
WonLab
|
| Street address |
3400 Civic Center Blvd
|
| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA295297 |
| SRA |
SRP063563 |
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