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| Status |
Public on Jan 01, 2016 |
| Title |
Self-maintenance of rat C6 glioma stem cells (GSCs) by their non-GSC progenies |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of Hoechst 33342 dye-effluxing side population cells (SP cells defined as glioma stem cells, GSCs) and dye-retaining main population cells (MP cells defined as non-GSCs) that were FACS-sorted from the C6 glioma cell line stably expressing EGFP (C6-eGFP). ECM-related genes, such as Col4a1 and Col4a2, and the iron carrier gene Tf are upregulated in MP cells. Results provide the insight into molecular basis underlying the maintenance of GSCs by non-GSCs.
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| Overall design |
Gene expression profiles were compared between SP and MP cells just after FACS-sorting from the whole C6-eGFP cells based on their Hoechst-effluxing abilities.
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| Contributor(s) |
Tabu K, Taga T |
| Citation(s) |
26822103 |
| |
| Submission date |
Aug 27, 2015 |
| Last update date |
Feb 08, 2016 |
| Contact name |
Kouichi Tabu |
| E-mail(s) |
k-tabu.scr@mri.tmd.ac.jp
|
| Organization name |
Tokyo Medical and Dental University
|
| Street address |
1-5-45, Yushima, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
1138510 |
| Country |
Japan |
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| Platforms (1) |
| GPL7294 |
Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA294049 |
