|
| Status |
Public on Aug 27, 2015 |
| Title |
Transcriptomic effects of Capridine on the acute promyelocytic leukemia HL-60 cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We used Agilent human gene expression arrays to test whether Capridine, a chemotherapeutic agent with specificity towards prostate cancer, deregulates the genomic fabric of acute myeloyd leukemia in HL-60 cells. This cell line was derived at the National Cancer Institute from a 36-year-old woman with acute promyelocytic leukemia. We found that Capridine down-regulates the pathways responsible for formation of hematopoietic progenitor cells, for anti-apoptosis and for proliferation of clonal neoplastic cells. Our results indicates the therapeutic potential of Capridine against acute myeloid leukemia.
|
| |
|
| Overall design |
Two-conditions: HO = not treated, HA = Capridine-treated HL-60 cells. Four biological replicates of each condition. Differently labeled biological replicates were cohybridized with each array. Results of similarly labeled different conditions were compared then averaged for the two fluorescent labels. For instance: HO1 & HO3 were compared with HA1 & HA3, HO2 & HO4 were compared with HA2 & + HA4, and the results of the comparisons averaged. This design uses 100% of the resources, has a better normlaiztion and allows all possible comparisons among the conditions.
|
| |
|
| Contributor(s) |
Iacobas DA, Suriano R, Mukherjee P, Gupta T, Gupta A, Iacobas S, Fallon JT, Geliebter J, Mittelman A, Tiwari RK |
| Citation(s) |
31349573 |
| |
| Submission date |
Aug 26, 2015 |
| Last update date |
Aug 14, 2019 |
| Contact name |
Dumitru Andrei Iacobas |
| E-mail(s) |
daiacobas@pvamu.edu
|
| Phone |
936-261-9926
|
| Organization name |
Prairie View A&M University
|
| Department |
Undergraduate Medical Academy
|
| Lab |
Personalized Genomics
|
| Street address |
Ann Preston St
|
| City |
Prairie View |
| State/province |
TX |
| ZIP/Postal code |
77446 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10332 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA293999 |
Less...
More...