|
| Status |
Public on Aug 12, 2015 |
| Title |
Research resource: global identification of estrogen receptor β target genes in triple negative breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this work was to identify all estrogen receptor beta target genes using RNA sequencing in MDA-MB-468 triple negative breast cancer cells engineered with inducible expression of full length estrogen receptor beta.
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| |
|
| Overall design |
MDA-MB-468 breast cancer cells with inducible ERb expression (MDA-468-ERb cells) were treated in triplicate with vehicle (control, no ERb) or doxycycline (plus ERb) for 48 hr prior to treatment with 0.1% DMSO vehicle or 10 nM 17b-estradiol for 4 hr.
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| |
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| Contributor(s) |
Shanle EK, Xu W |
| Citation(s) |
23979844 |
| |
| Submission date |
Aug 11, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Erin K Shanle |
| E-mail(s) |
eshanle7@gmail.com
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Biochemistry and Biophysics
|
| Lab |
Brian D. Strahl
|
| Street address |
3010 Genetic Medicine, 120 Mason Farm Road
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA292572 |
| SRA |
SRP062287 |
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