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Series GSE7181 Query DataSets for GSE7181
Status Public on Mar 06, 2007
Title CD133+ and CD133- CSC lines in primary human GBM
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We cultured tumor cells from 22 GBM under medium conditions favoring the growth of neural stem cells. 11 out of 15 primary GBM contained a significant CD133+ subpopulation that comprised cells showing all hallmarks of neural stem cells. Cell lines derived from these CD133+ GBM showed a neurosphere-like, non-adherent growth pattern. In contrast, 4 out of 15 cell lines derived from primary GBM grew adherent in vitro and were driven by CD133- tumor cells that fulfilled stem cell criteria. In vivo, these GBM were characterized by a significantly lower proliferation index but similar GFAP staining as compared to CD133+ GBM. Gene arrays from 2x3 representative cells lines are given.
Keywords: Cancer stem cell, CD133, glioblastoma
 
Overall design Human glioblastoma cells cultured in DMEM supplemented with EGF, FGF, LIF, B27.
 
Contributor(s) Beier CP, Beier D, Bogdahn U, Oefner PJ
Citation(s) 17483311
Submission date Mar 03, 2007
Last update date Mar 25, 2019
Contact name Christoph P. Beier
Organization name University of Regensburg
Department Neurology
Lab NeuroOncology
Street address Universitatsstrasse 84
City Regensburg
ZIP/Postal code 93042
Country Germany
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM170898 Adherent Glioma Cell Line R8
GSM172063 Neurosphere-like glioma Cell Line R11
GSM172064 Neurosphere-like glioma Cell Line R18
Relations
BioProject PRJNA98331

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE7181_RAW.tar 31.0 Mb (http)(custom) TAR (of CEL, CHP)
Processed data provided as supplementary file

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