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| Status |
Public on Feb 26, 2016 |
| Title |
Expression data from E12.5 male fatal PGCs, PGCLC in vitro, round spermatids and spermatids like cells produced in vitro |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Generation of haploid gametes in vitro can potentially address gamete failure-based infertility.This study reports complete in vitro meiosis from murine ESC-derived PGCLCs resulting in the formation of male spermatid-like cells (SLCs) capable of producing viable fertile offspring via intracytoplasmic sperm injection (ICSI).Our findings provide the basis for generation of haploid spermatids in vitro in human, the generation of transgenic animals, and the use of this system to investigate mechanisms of meiosis.
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| Overall design |
We used microarrays to compare gene expression profiles of in vivo and in vitro derived PGC cells and round spermatids.
We collected E12.5 male fatal PGCs, PGCLC in vitro, round spermatids and spermatids like cells produced in vitro, each sample has 3 replications.
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| Contributor(s) |
Zhou Q, Sha J, Zhao X |
| Citation(s) |
26923202 |
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| Submission date |
Jul 29, 2015 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Quan Zhou |
| E-mail(s) |
zhouquan0807@foxmail.com
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| Organization name |
Nanjing Medical University
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| Street address |
NO. 140, Hanzhong Road
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210000 |
| Country |
China |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA291354 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE71478_RAW.tar |
47.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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