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| Status |
Public on Aug 25, 2015 |
| Title |
Expression array analysis of the hepatocyte growth factor (HGF) invasive program |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
HGF stimulates mitogenesis, motogenesis and morphogenesis in most epithelial target cells. Selective inhibition of HGF signaling blocks spontaneous metastasis, but not primary tumor growth, in the prostate adenocarcinoma derived PC3M cell xenograft model.
To identify the HGF activated genes and pathways that contribute to cell motility and invasion, i.e. the HGF invasive program, expression profiling was performed on PC3M cells that were untreated or were treated with human HGF (hHGF), which drives all 3 primary activities, or with human HGF/NK2 (hNK2) or murine HGF (mHGF), which drive motility and invasion but not proliferation in human cells.
Differences in gene expression profiles among these four groups were used to distiniguish between events associated with invasion (resulting from hNK2 or mHGF treatment) from those associated with the combination of invasion and proliferation (resulting from hHGF treatment).
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| Overall design |
PC3M cells were left untreated or treated with hHGF, hNK2 or mHGF for 8, 16 or 32 hours prior to RNA extraction, cDNA preparation and array hybridization.
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| Contributor(s) |
Cecchi F, Lih C, Lee YH, Walsh W, Rabe DC, Williams PM, Bottaro DP |
| Citation(s) |
26231668 |
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| Submission date |
Jul 27, 2015 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Donald P Bottaro |
| E-mail(s) |
dbottaro@helix.nih.gov
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| Organization name |
National Cancer Institute
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| Department |
Center for Cancer Research
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| Lab |
Urologic Oncology Branch
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| Street address |
Bldg 10 CRC Rm 2-3952, 10 Center Drive
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (36)
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| GSM1833761 |
PC3M treated with 1 nM Human HGF for 16 hr, biological replicate 1 |
| GSM1833762 |
PC3M treated with 1 nM Human HGF for 16 hr, biological replicate 2 |
| GSM1833763 |
PC3M treated with 1 nM Human HGF for 16 hr, biological replicate 3 |
| GSM1833764 |
PC3M treated with 1 nM Human HGF for 32 hr, biological replicate 1 |
| GSM1833765 |
PC3M treated with 1 nM Human HGF for 32 hr, biological replicate 2 |
| GSM1833766 |
PC3M treated with 1 nM Human HGF for 32 hr, biological replicate 3 |
| GSM1833767 |
PC3M treated with 1 nM Human HGF for 8 hr, biological replicate 1 |
| GSM1833768 |
PC3M treated with 1 nM Human HGF for 8 hr, biological replicate 2 |
| GSM1833769 |
PC3M treated with 1 nM Human HGF for 8 hr, biological replicate 3 |
| GSM1833770 |
PC3M treated with 1nM Mouse HGF for 16 hr, biological replicate 1 |
| GSM1833771 |
PC3M treated with 1nM Mouse HGF for 16 hr, biological replicate 2 |
| GSM1833772 |
PC3M treated with 1nM Mouse HGF for 16 hr, biological replicate 3 |
| GSM1833773 |
PC3M treated with 1nM Mouse HGF for 32 hr, biological replicate 1 |
| GSM1833774 |
PC3M treated with 1nM Mouse HGF for 32 hr, biological replicate 2 |
| GSM1833775 |
PC3M treated with 1nM Mouse HGF for 32 hr, biological replicate 3 |
| GSM1833776 |
PC3M treated with 1nM Mouse HGF for 8 hr, biological replicate 1 |
| GSM1833777 |
PC3M treated with 1nM Mouse HGF for 8 hr, biological replicate 2 |
| GSM1833778 |
PC3M treated with 1nM Mouse HGF for 8 hr, biological replicate 3 |
| GSM1833779 |
PC3M treated with 30 nM NK2 for 16 hr, biological replicate 1 |
| GSM1833780 |
PC3M treated with 30 nM NK2 for 16 hr, biological replicate 2 |
| GSM1833781 |
PC3M treated with 30 nM NK2 for 16 hr, biological replicate 3 |
| GSM1833782 |
PC3M treated with 30 nM NK2 for 32 hr, biological replicate 1 |
| GSM1833783 |
PC3M treated with 30 nM NK2 for 32 hr, biological replicate 2 |
| GSM1833784 |
PC3M treated with 30 nM NK2 for 32 hr, biological replicate 3 |
| GSM1833785 |
PC3M treated with 30 nM NK2 for 8 hr, biological replicate 1 |
| GSM1833786 |
PC3M treated with 30 nM NK2 for 8 hr, biological replicate 2 |
| GSM1833787 |
PC3M treated with 30 nM NK2 for 8 hr, biological replicate 3 |
| GSM1833788 |
PC3M untreated for 16 hr, biological replicate 1 |
| GSM1833789 |
PC3M untreated for 16 hr, biological replicate 2 |
| GSM1833790 |
PC3M untreated for 16 hr, biological replicate 3 |
| GSM1833791 |
PC3M untreated for 32 hr, biological replicate 1 |
| GSM1833792 |
PC3M untreated for 32 hr, biological replicate 2 |
| GSM1833793 |
PC3M untreated for 32 hr, biological replicate 3 |
| GSM1833794 |
PC3M untreated for 8 hr, biological replicate 1 |
| GSM1833795 |
PC3M untreated for 8 hr, biological replicate 2 |
| GSM1833796 |
PC3M untreated for 8 hr, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA291099 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE71404_RAW.tar |
165.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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