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| Status |
Public on Oct 01, 2015 |
| Title |
A mechanism for expansion of the regulatory T cell repertoire and its role in enforcing self-tolerance. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Thymic Treg cells, mature non-Treg CD4+ single positive thymocytes, peripheral (spleen) resting and activated Treg cells were sorted from Foxp3-gfp reporter (wid type, WT) mice or Foxp3 enhancer CNS3 knockout (KO, carrying the same GFP reporter) mice. Total RNA was extracted and used for RNA sequencing to assess gene expression profiles.
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| Overall design |
Two 6-8 week old littermates of male Foxp3-gfp and Foxp3ΔCNS3-gfp mice were used to sort Treg cells and conventional CD4+ T cells. Lymphocyte preparation and electronic sorting were performed at the same time. RNA extraction, SMART amplification, library preparation were conducted in parallel.
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| Contributor(s) |
Feng Y, Osmanbeyoglu HU, Rudensky AY |
| Citation(s) |
26605529 |
| |
| Submission date |
Jul 24, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Hatice Ulku Osmanbeyoglu |
| Organization name |
University of Pittsburgh
|
| Street address |
5117 Centre Ave
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA290884 |
| SRA |
SRP061546 |
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